缺血再灌注损伤大鼠肾脏凝血纤溶相关蛋白的表达

目的 观察缺血再灌注(IR)大鼠肾组织凝血纤溶相关物质的蛋白表达并探讨其意义.方法 雄性Wistar大鼠48只被随机分为6组:假手术组(Sham组)、缺血再灌注0、2、6、12、24 h组(IR0h组、IR2h组、IR6h组、IR 12h组、IR 24 h组),每组8只.用无创动脉夹夹闭双肾动脉45 min建立IR模型.HE染色观察肾组织病理改变；免疫组织化学法检测肾组织内皮素(ET-1)、组织型纤溶酶原激活物(tPA)、纤维蛋白原样蛋白2(Fgl-2)凝血酶原酶蛋白的表达.结果 与Sham组相比,IR 24 h组大鼠血BUN及Scr升高,差异有统计学意义(P＜0.05).IR各组肾组织病理改变程度随再灌注时间延长而逐渐加重；与Sham组相比,IR各组肾小管损伤半定量评分明显升高,差异有统计学意义(P＜0.05).IR各组肾ET-1蛋白表达增加,6h达高峰,与Sham组相比差异有统计学意义(P＜0.05).IR各组肾Fgl-2凝血酶原酶表达增加,12h达高峰,24 h基本恢复正常,与Sham组相比,差异有统计学意义(P＜0.05).肾组织t-PA表达增加,12h达高峰,与Sham组相比,差异有统计学意义(P＜0.05).结论 肾脏IR损伤后存在肾组织凝血纤溶相关物质的异常表达,推测后者可能参与了肾脏IR损伤.

Expression of related substances of coagulation and fibrinolysis in the rat kidneys with ischemia reperfusion injury

Objective To investigate the expression and significance of related substances of coagulation and fibrinolysisintheratkidneyswithischemiareperfusion(IR)injury. Methods Forty-eight male Wistar rats were randomly divided into six groups: Sham, IR 0 h, IR 2 h, IR 6 h, IR 12 h and IR 24 h. IR rats were made by occlusion of bilateral renal arteries by bulldog clamp for 45 minutes. Renal pathology was observed by HE staining. The expression of endothelin-1（ET-1）, tissue plasminogen activator (t - PA) and fibrinogen - like protein 2 (Fgl - 2) of renal tissue was detected by immunohistochemical method. Results Scr and BUN increased significantly in IR 24 h group compared with sham group(P＜0.05). Renal pathological injury was significantly aggravated with prolonged reperfusion. The semi- quantitative scores of renal tubular injury were increased in all IR group compared with sham group(P＜0.05). The expression of ET-1 was gradually increased before the first six hour after IR and decreased after twelve hour. Compared with sham group, ET-1 was increased significantly in all IR group. The expression of Fgl-2 fibroleukin in renal tissue increased progressively in the first six hour after IR, weaken in IR 12 h group and trended to normal in IR 24 h group. The expression of t-PA increased progressively during the first six hour after IR, maintained this level in IR 12 h group and decreased in IR 24 h group. Conclusion The abnormal expression of related substances of coagulation and fibrinolysis of rats may participate in renal IR injury.