NSCLC患者血清P16基因甲基化与P53抗体水平检测的临床诊断价值

目的 探讨血清P16基因甲基化与P53抗体水平的检测对非小细胞肺癌(NSCLC)的诊断价值和临床意义.方法 选择98例NSCLC患者为实验组,以60名健康者作为对照,分别采用甲基化特异性PCR法检测其血清P16基因甲基化,用酶联免疫吸附试验法检测其血清P53抗体.结果 P16基因甲基化和P53抗体定性检测对NSCLC预测诊断的敏感度分别为70.41％和58.16％,特异度分别为85.00％和93.33％,正确指数为0.554和0.515;P16基因甲基化在NSCLC患者和健康对照者血清中阳性率分别为70.41％和15.00％ (x2=45.709,P＜0.001);P53蛋白抗体在NSCLC患者和健康对照者血清中阳性率分别为58.16％和6.67％(x2=41.638,P＜0.001);对NSCLC诊断做ROC曲线结果显示血清学P53抗体定量检测对NSCLC预测诊断有统计学意义;P16基因甲基化和P53抗体定性联合检测结果显示对NSCLC诊断的敏感度为82.56％,特异度为78.33％,正确指数为0.610.P16基因甲基化和P53蛋白抗体联合检测的敏感度高于单独检测(x2=14.105,P=0.001).结论 血清中P16基因甲基化与P53抗体的检测对NSCLC患者的预测诊断具有实际意义,联合检测的敏感度高于单独检测,可在临床上予以推广.

Clinical diagnosis value of P16 gene methylation and P53 antibody in serum of non-small cell lung cancer patients

Objective To investigate the clinical diagnosis value of P16 gene methylation and P53 antibody in serum for non-small cell lung cancer (NSCLC).Methods The P16 gene methylation and P53 antibody level in serum from 98 NSCLC patients and 60 normal controls were tested with methylationspecific polymerase chain reaction and enzyme linked immunosorbent assay.Results The sensitivities of P16 gene methylation and P53 antibody qualitative detection were 70.41％ and 58.16％ for prediction diagnosis of NSCLC,the specificities were 85.00％ and 93.33％,the correct indexes were 0.554 and 0.515.The positive rates of P16 gene methylation in serum of NSCLC patients and healthy controls were 70.41％ and 15.00％ (x2 =45.709,P ＜0.001).The positive rates of P53 antibody in serum of NSCLC patients and healthy controls were 58.16％ and 6.67％ (x2=41.638,P ＜0.001).The result of receiver operating characteristic curve in the diagnosis of NSCLC showed that the quantitative detection of serum P53 antibody in the diagnosis of NSCLC was statistically significant.The sensitivity and specificity of combined detection of P16 methylation and P53 antibody were 82.56％ and 78.33％ for NSCLC diagnosis,the correct index was 0.610.The sensitivity of combined detection of P16 methylation and P53 antibody in NSCLC was higher than that of single detection (x2=14.105,P =0.001).Conclusions The detection of P16 gene methylation and P53 antibody in serum of NSCLC patients is of practical significance.The sensitivity of combined detection is higher than that of single detection.