雌激素干预对去卵巢大鼠骨组织的影响

目的:建立绝经后骨质疏松(PMO)模型,外源性补充雌激素,探讨雌二醇(E_2)的骨保护作用及其对胞质活化T细胞核因子1(NFATc1)表达的影响。方法:12周龄Wistar雌性大鼠随机分为假手术组(Sham组)、去势组(OVX组)、实验组(OVX+E_2组),每组10只。OVX+E__2组给予17β-E_2皮下注射,其余2组给予等量生理盐水。16周后,酶联免疫吸附试验(ELISA)测定血清E_2水平,逆转录聚合酶链反应(RT-PCR)法测定骨组织中NFATc1表达量,微型计算机断层成像(Micro-CT)分析大鼠胫骨平台处骨密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)。结果:Sham组、OVX+E_2组与OVX组相比,BMD、Tb.Th、Tb.N、BV/TV、血清E_2水平升高,NFATc1水平下降(均P0.05)。结论:雌激素可能通过抑制NFATc1的表达减缓骨量丢失。

Effects of Estrogen on Osteoporosis in Ovariectomized Rats

Objective:To investigate the protective effects of exogenous estrogen on bone tissue in a postmenopausal osteoporosis (PMO) rat mode,as well as the effect on the expression of NFATc1.Methods:The 12 weeks Wistar rats were randomly divided into sham operation group,OVX group,and OVX+E2 group and 10 rats in each group.In the OVX+E2 group,17β-F2 was injected subcutaneously and the other two groups were given the same volume of normal saline.After 16 weeks,the serum E2 level was determined by ELISA,the expression of NFATc1 in bone tissue were measured by RT-PCR,the bone mineral density (BMD),the fraction of bone tissues (BV/TV),the thickness of the trabecular bone (Tb.Th) and number of trabecular bone (Tb.N) were measured using Micro-CT analysis.Results:The OVX+E2 group and sham operation group compared with the OVX group,the levels of BMD,BV/TV,Tb.Th,Tb.N and serum E2 were significantly increased,the level of NFATc1 were significantly decreased (P＜0.05).Conclusions:Estrogen may prevent bone loss by decreasing NFATc1 expression in OVX rats.