清道夫受体A在COPD发病及进展为肺癌中的研究

目的 探讨清道夫受体A基因多态性与COPD发病及进展为肺癌的关系.方法 选择2016年1月1日至6月20日上海交通大学医学院附属瑞金医院单纯COPD患者、COPD合并肺癌患者和健康吸烟对照者各100例,抽取外周血进行基因测序,检测SR-A 8个单核苷酸多态性[(single nucleotide polymorphism,SNP):ex3P36A_ C＞G、ex3S41Y～C＞A、ex4V113A～T＞C、ex4P174Y～G＞T、ex6P275A C＞G、ex6R293X C＞T、ex10G369S_ G＞A和ex11H441R_ A＞G].结果 上述8个SNP在3组人群中差异无统计学意义,但在基因测序过程中发现新的位于内含子剪接区的SNPrs13306550 (IVS4+ 3A＞G)与COPD发病相关,但对COPD进展为肺癌影响不大.结论 本研究表明,清道夫受体A SNP rs13306550是COPD的一个危险因素,但对COPD患者进展为肺癌的影响较小.

Role of macrophage scavenger receptor 1 gene polymorphisms in COPD with and without lung cancer

Objective To investigate the relationship between the variants of the class A scavenger receptor (SR-A) and chronic obstructive pulmonary disease (COPD) with or without lung cancer in China.Methods From January 1,2016,to June 20,2016,100 patients with COPD with lung cancer,100 patients with COPD without lung cancer,and 100 healthy smokers were enrolled into Shanghai Ruijin Hospital for genotyping of eight single nucleotide polymorphisms (SNPs:ex3P36A_C＞G,ex3S41Y_C＞A,ex4V113A_T＞ C,ex4P174Y_G＞ T,ex6P275A_C＞ G,ex6R293X_C＞ T,ex10G369S_G＞ A,and ex11H441R_A＞G) by gene sequencing.Results The genotype frequencies of the aforementioned eight SNPs between patients with COPD with and without lung cancer and healthy controls were not significantly different.However,during DNA sequencing,a new promising SNP,rs13306550 (IVS4 +3A ＞G),was found in the splice donor site,which was significantly associated with an increased risk of COPD.Conclusions This study demonstrated that the variant rs13306550 was a risk factor for COPD susceptibility,but had little influence on the pathogenesis of lung cancer in patients with COPD.