香烟烟雾致COPD模型大鼠气道纤毛结构变化的评价

目的 应用气道纤毛超微结构的综合评价方法,观察香烟烟雾暴露对COPD大鼠气道纤毛结构的影响.方法 Wister大鼠,随机分为对照组(5只)、香烟暴露组(5只),香烟暴露组通过45 d被动吸烟建立COPD大鼠模型,利用光镜观察肺组织病理学变化,电镜观察纤毛超微结构改变,免疫荧光标记技术观察纤毛超微结构中微管蛋白变化.结果 香烟暴露组大鼠的纤毛荧光强度为(53.46±13.28),低于对照组(120.00±26.86)(P＜0.05).香烟暴露组纤毛膜水疱数为(7.36±2.38)个高于对照组(O.66±1.21)个(P＜0.05).结论 被动吸烟导致COPD大鼠气道纤毛结构异常,表现为纤毛膜水疱数增加、纤毛荧光强度减低.

Evaluation of changes in airway ciliary structure in COPD model rats induced by cigarette smoke

Objective The effects of cigarette smoke exposure on airway ciliary structure in COPD rats were evaluated by the comprehensive evaluation of airway cilia ultrastructure.Methods Wister rats were randomly divided into two experimental groups (CON:control group,n =5;CS:cigarette smoke group,n =5).Rats in CS group were exposed to passive smoking for 45 days.The abnormalities of ciliary ultra-structures and the fluorescence intensity of ciliated microtubules were measured by electron microscopy (EM) and confocal microscopy.Results The ciliary ultrastructure abnormalities were found in CS group with typical cilia membrane vesiculation and dynein arm deficiency under EM.The vesiculation in CS group significantly increased (7.36±2.38) compared with CON group (0.66±1.21).Meanwhile,the microtubule of cilia in CS group decreased,with immune fluorescence intensity was (53.46 ± 13.28),compared to CON group (120.00±26.86)(P ＜0.05).Conclusions Cigarette smoking could affect the ciliated epithelium in smoking induced airway rats model,with ciliary ultrastructural abnormalities and absence of cilia microtubules.