The differential activities of R (+)- and S(-)-zacopride as 5-HT3 receptor antagonists |
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Authors: | J M Barnes N M Barnes B Costall A M Domeney D N Johnson M E Kelly H R Munson R J Naylor R Young |
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Institution: | Postgraduate Studies in Pharmacology, School of Pharmacy, University of Bradford, West Yorkshire, UK. |
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Abstract: | R(+)- and S(-)-zacopride were assessed as potential 5-HT3 receptor antagonists in behavioural and biochemical tests. The S(-)isomer was more potent than the R(+)isomer to antagonise the hyperactivity induced by the injection of amphetamine or the infusion of dopamine into the nucleus accumbens in the rat. In contrast, the R(+)isomer was more potent to reduce the aversive behaviour of mice to a brightly illuminated environment and in a marmoset human threat test, to facilitate social interaction in rats, to increase performance in a mouse habituation test and prevent a scopolamine-induced impairment, and to antagonise the inhibitory effect of 2-methyl-5-hydroxytryptamine to reduce 3H]acetylcholine release in slices of the rat entorhinal cortex. In binding assays, 3H]S(-)-zacopride and 3H]R(+)-zacopride labelled homogenous populations of high-affinity binding sites in the rat entorhinal cortex, R(+)-zacopride compete for a further 10 to 20% of the binding of 3H]R(+)/S(-)-zacopride or 3H]R(+)-zacopride in excess of that competed for by (S)(-)-zacopride. It is concluded that both isomers of zacopride have potent but different pharmacological activities, with the possibility of different recognition sites to mediate their effects. |
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