大鼠自体移植脾组织GAP-43+神经再生的实验研究

目的 研究大鼠自体脾组织移植术后移植脾组织GAP-43^ 神经纤维再生及分布规律。方法 健康Wistar大鼠105只，随机分为自体脾组织移植组和对照组，于术后7、15、30、60、90、120、180d取移植脾组织标本，应用免疫组化方法显示GAP-43^ 神经纤维，并用图像分析系统，对不同时相点免疫组化GAP-43^ 染色区域进行图像定量分析。结果 自体脾组织移植术后30d，移植脾组织周围大网膜内可见GAP-43^ 神经纤维，并向移植脾组织内伸展；自体脾组织移植术后60～120d，移植脾组织内再生神经纤维逐渐增多；术后180d，移植脾组织中GAP-43^ 神经纤维分布及密度接近正常。结论GAP-43^ 神经纤维于脾移植术后60d开始出现，术后180dGAP-43^ 神经纤维接近正常脾。

Experimental study on regeneration of GAP-43 positive nerve fibers in splenic autotransplantation

Objective To explore the regeneration of growth associated protein 43 (GAP-43) positive nerve fibers in splenic autotransplantation in rats. Methods Healthy Wistar rats were randomly divided into two groups: splenectomy plus splenic autotransplantation group, and sham-operation group. The regeneration of GAP-43 positive nerve fibers was observed by immunohistochemical method at 7, 14, 30, 60, 90, 120, and 180 d after operation. Computer image analysis was conducted for analysis of the density changes. Results The GAP-43 nerve fibers appeared in the greater omentum at 30 d after operation, and the density of nerve fibers became greater at 60-120 d and became almost normal on 180 d after operation. Conclusion GAP-43 nerve fibers are found in the autotransplant tissues at 60 d and become almost normal at 180 d after splenic autotransplantation.