| 去糖基化改变rNav1．3门控性质：糖基化／去糖基化的动态平衡可能使电压门控钠通道的调控功能更具复杂性 |
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| 引用本文： | 徐清,程慧雯,何慧琼,刘志睿,贺明,杨宏天,周智磊,吉永华.去糖基化改变rNav1．3门控性质：糖基化／去糖基化的动态平衡可能使电压门控钠通道的调控功能更具复杂性[J].神经科学通报,2008,24(5):283-287. |
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| 作者姓名： | 徐清 程慧雯 何慧琼 刘志睿 贺明 杨宏天 周智磊 吉永华 |
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| 作者单位： | [1]上海大学生命科学学院,上海200444 [2]中国科学院上海生命科学研究院,上海200031 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的 观察糖基化对rNav1.3电压门控性质的影响.方法 将rNav1.3在爪蟾卵母细胞中表达,衣霉素抑制其糖基化,双电极电压钳记录全细胞电流,Origin软件分析处理数据.对照组为未去糖基化的rNav1.3.结果 与糖基化的rNav1.3相比,去糖基化的rNav1.3稳态激活曲线向去极化方向偏移约10 mV,稳态失活曲线向超极化方向偏移约8 mV.结论 糖基化修饰改变rNav1.3的电压门控性质,异化其生理功能.
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| 关 键 词： | 电压门控钠通道 糖基化 双电极电压钳 爪蟾卵母细胞 |
Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel |
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| Qing Xu,Hui-Wen Cheng,Hui-Qiong He,Zhi-Rui Liu,Ming He,Hong-Tian Yang,Zhi-Lei Zhou,Yong-Hua Ji.Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel[J].Neuroscience Bulletin,2008,24(5):283-287. |
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| Authors: | Qing Xu Hui-Wen Cheng Hui-Qiong He Zhi-Rui Liu Ming He Hong-Tian Yang Zhi-Lei Zhou Yong-Hua Ji |
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| Institution: | School of Life Science, Shanghai University, Shanghai 200444, China. |
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| Abstract: | OBJECTIVE: To examine the effect of deglycosylation on gating properties of rNav1.3. METHODS: rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. RESULTS: Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. CONCLUSION: Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity. |
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| Keywords: | rNav1 3 rNavl 3 voltage-gated sodium channel glycosylation two-electrode voltage clamp Xenopus oocyte |
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