孟鲁司特对幼年哮喘大鼠气道重塑的影响

目的:探讨孟鲁司特对幼年哮喘大鼠气道重塑的影响.方法:幼年SD大鼠随机分为模型组、时照组、孟鲁司特组,每组8只.应用卵蛋白致敏、激发8周建立哮喘气道重塑模型,孟鲁司特组每次激发前2h给予孟鲁司特15 mg/kg灌胃,采用HE染色和计算机图像分析系统测定各组大鼠支气管壁及平滑肌面积,应用样本碱水解法测定羟脯氨酸含量.结果:(1)模型组Wat/Pbm及Wam/Pbm分别为(72.53±5.71) μm和(24.57+2.51)μm,孟鲁司特组Wat/Pbm及Wam/Pbm分别为(63.17≈4.99)μm和(16.31±2.14)μm,与模型组比较明显减轻,差异有统计学意义(P＜0.05)；(2)模型组羟脯氨酸含量为(1 224.51 +99.64) μg/g肺组织涅重,高于对照组含量(786.51±78.95)μg/g肺组织湿重,差异有统计学意义(P＜0.01)；(3)孟鲁司特组羟脯氨酸含量为(1064.90±86.76)μg/g肺组织湿重,较模型组含量明显减少,差异有统计学意义(P＜0.01).结论:孟鲁司特能显著抑制气道壁平滑肌的增厚、胶原沉积.

The Effect of Montelukast Remodeling in Asthmatic Airway in Rats

Objective: To explore the effect of montelukast in asthmatic airway remodeling in rats.Methods: Twenty four SD rats were randomly divided into three groups: model group,control group and montelukast treatment group.Model group and montelukast treatment group were administrated with Ovalbumin for 8 weeks,however,montelukast treatment group were given montelukast 15 mg/kg through gastric lavage 2 hours before Ovalbumin sensitization.HE staining and image analysis were used to measure Wat/Pbm and Wam/Pbm,and hydroxyproline content were determined in different groups.Results:(1) The Wat/Pbm and Wam/Pbm,in the model group were(72.53±5.71) μm and(24.57±2.51) μm,and in montelukast treatment group were(63.17±4.99) μm and(16.31±2.14) μm.Either in model group or montelukast treatment group,the Wat/Pbm and Wam/Pbm,were obvious increased than those in control group(P<0.05).However,the Wat/Pbm and Wam/Pbm in montelukast treatment group were lesser than those in model group(P<0.05);(2) Hydroxyproline contents of lung tissue in both model and montelukast treatment group were increased than those in control group(P<0.01),and in model group were even higher(P<0.01).Conclusions: Montelukast can inhibit the airway smooth muscle thickening and collagen deposition.