| 视网膜下液对培养的人眼视网膜色素上皮细胞和成纤维细胞增殖作用的影响 |
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| 引用本文： | 吴苗琴,王竞. 视网膜下液对培养的人眼视网膜色素上皮细胞和成纤维细胞增殖作用的影响[J]. 眼科研究, 2001, 19(1): 29-31 |
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| 作者姓名： | 吴苗琴 王竞 |
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| 作者单位： | 1. 浙江大学第一附属医院浙益眼科中心 杭州，310006;浙江省人民医院眼科
2. 浙江大学第一附属医院浙益眼科中心 杭州,310006 |
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| 摘 要： | 目的：了解不同程度增生性玻璃体视网膜病变（PVR）的视网膜下液（SRF）对视网膜色素上皮细胞（RPE）、成纤维细胞（FB）增殖的影响。方法：用溴标法和MTT法测定不同程度PVR的SRF对RPE和FB增殖的促进作用。结果：几乎所有的SRF都具有促进细胞增殖的作用，用1：10比例稀释，PVR程度为PVR＜C1，PVRC1，PVR＞C13组。SRF作用24h后RPE的增殖率分别为对照组的128.5％，139.8％，156.8％，FB的增殖率分别为对照组的126.3％，143.1％及172.3％。结论：SRF液促进细胞增殖的作用随着PVR程度的加重而增强。
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| 关 键 词： | 增生性玻璃体视网膜病变 视网膜下液 色素上皮细胞 成纤维细胞 PVR |
| 修稿时间： | 2000-02-23 |
Effects of subretinalfluid on proliferation of cultured human retinal pigment epithelial cells and fibroblastcells |
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| Wu Miaoqin,Wang Jing. Effects of subretinalfluid on proliferation of cultured human retinal pigment epithelial cells and fibroblastcells[J]. Chinese Ophthalmic Research, 2001, 19(1): 29-31 |
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| Authors: | Wu Miaoqin Wang Jing |
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| Affiliation: | Wu Miaoqin, Wang Jing Department of Ophthalmology,First Affiliated Hospital,Zhejiang University,Hangzhou 310006 |
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| Abstract: | Objective To detect the effect of subretinal fluid (SRF) from different grades of proliferative vitreoretinopathy (PVR) on cultured retinal pigmental epithelial cells(RPE) and fibloblasts(FB). Methods The effect of SRF from different grades of PVR on RPE and FB was determined using Brdu labeling technique and MTT assaying method,respectively. Results All samples showed a stimulating effect on the two kinds of cellular elements in a certain degree. At l: 10 concentration,the cellular proliferation stimulating activity of SRF with PVR < C1, PVR C, and PVR > C, on RPE and FB was 128. 5%, 139. 8%, 156. 8% and 126. 3%, 143. 1 %, 172. 3% of the control groups, respectively after 24 h stimulation. Conclusions SRF's proliferation-stimulating activity is correlated with the grades of PVR. |
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| Keywords: | proliferative vitreoretinopathy subretinal fluid retinal pigment epithelial cells fibroblast |
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