| HPLC法测定乌红天麻中天麻素的含量 |
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| 引用本文： | 王福霞,魏清.HPLC法测定乌红天麻中天麻素的含量[J].中国医药指南,2007(5):93-94. |
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| 作者姓名： | 王福霞 魏清 |
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| 作者单位： | 1. 湖北省宜昌市第一人民医院
2. 湖北省宜昌市中心医院 |
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| 摘 要： | 目的建立乌红天麻中天麻素的含量测定方法。方法采用高效液相色谱法,色谱柱为NUCLEOSILC_(18)(4.6mm×250mm,5(?)m),流动相为乙晴:水(3:97),流速为1mL·min~(-1),检测波长为220nm,柱温为25℃。结果乌红天麻中天麻素在10.83～64.98(?)g·mL~(-1)内呈良好的线性关系,回归方程为A=103.7C+2.4r=0.9999;平均回收率为100.1%,RSD=0.3%。结论本法快速、简便、准确,可用于乌红天麻药材的质量控制。
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| 关 键 词： | HPLC色谱 乌红天麻 天麻素 含量 |
HPLC determination of Gastrodin in WUhongtianma |
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| WANG Fuxia,WEI qing.HPLC determination of Gastrodin in WUhongtianma[J].Guide of China Medicine,2007(5):93-94. |
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| Authors: | WANG Fuxia WEI qing |
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| Abstract: | To establish an HPLC method for the determination of Gastrodin in Wuhongtianma.Methods:The column was NUCLEOSILC_(18)(4.6mm×250mm,5μm),the mobile phase consisted of Acetonitrile-Water(3:97),the flow rate was 1. 0mL·min~1,the detection wavelength was 220nm,the column temperature was 25℃. Results:The calibration curve was linear in the range of 10.83-64.98μg·mL~(-1)with r=0.9999,and the regression equation was A=103.7C+2.4(r=0.9999).The average recovery was 100.1%(RSD=0.3%).Conclusion:The method is rapid,accurate,sensitive for quality control of Gastrodin in Wuhongtianma |
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| Keywords: | HPLC wuhongtianma gastrodin content |
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