转酮酶样基因TKTL1在人类鼻咽癌发生和转移中的作用

目的 探讨转酮酶样基因TKTL1在人类鼻咽癌发生和转移中的作用.方法 对65例鼻咽癌和9例鼻咽部慢性炎症组织中转酮酶活性进行检测;实时定量PCR检测鼻咽癌和鼻咽部慢性炎症组织中转酮酶基因家族(TKT、TKTL1和TKTL2)mRNA表达水平的变化,并分析TKTL1的表达与鼻咽癌发生和转移的关系.结果 鼻咽癌组织中转酮酶活性较鼻咽部慢性炎症组织明显增强(t=4.12,P=0.00);实时定量PCR结果 示鼻咽癌和鼻咽部慢性炎症组织中TKT和TKTL2 mRNA的表达水平差异无统计学意义(t=1.28、1.62,P=0.32、0.26).然而,鼻咽癌组织中TKTL1的表达水平比鼻咽部慢性炎症组织明显上调(t=6.23,P=0.00),且有淋巴结转移的鼻咽癌组织中TKTL1的表达水平较尤淋巴结转移者明显增强,差异有统计学意义(t=3.28,P=0.00).结论 转酮酶样基因TKTL1在人类鼻咽癌的发生和转移中可能起重要作用,TKTL1有望成为肿瘤治疗研究的新靶点.

Effects of transketolase-like gene TKTL1 on occurrence and metastasis of human nasopharyngeal carcinoma

Objective To investigate the effects of transketolase-like gene TKTL1 on the occurrence and metastasis of human nasopharyngeal carcinoma (NPC). Methods Real-time PCR was used to determine the mRNA expression levels of transketolase gene family (TKT, TKTL1, and TKTL2) in the 65 biopsy specimens of human nasopharyngeal carcinoma, 9 at stage Ⅰ ,15 at stage Ⅱ , 16 at stage Ⅲ, 13 at stage Ⅳ A, and 12 at stage ⅣB, 42 with metastasis and 232 without metastasis, and 9 biopsy specimens of chronic nasopharyngitiss. Results The TKL activity level of the NPC tissues was 21.6±2.8, significantly higher than that of the chronic nasopharyngitis tissues (6.2±1.3, P <0. 01). The TKL activity level of the NPC tissues with metastasis was 26. 5±3.2, significantly higher than that of the NPC tissues without metastasis (17. 6 ± 2. 1, P < 0. 01). There was no significant difference in the expression levels of TKT and TKTL2 genes between the human NPC and chronic nasopharyngitis tissues (both P > 0. 05). The expression level of TKTL1 gene in the NPC tissues was 5.25±0. 32, significantly higher than that in the chronic nasopharyngitis tissues (0. 98±0. 11, t = 6. 23, P < 0. 01), and the expression level of TKTL1 gene in the NPC tissues with lymph node metastasis was 5.86±0. 38, significantly higher than that in the NPC tissues without lymph node metastasis (4. 18±0.22, t =3.28,P <0.01). Conclusion TKTL1 may play an important role in the occurrence and metastasis of human NPC and become a potential target for novel anti-cancer therapy.