益生菌对小鼠溃疡性结肠炎的影响

目的 探讨环氧合酶2(COX-2)在溃疡性结肠炎(UC)小鼠中的发病作用及益生菌治疗UC的可能作用机制.方法 采用右旋葡聚糖硫酸钠(DSS)诱导的小鼠UC模型,将60只BALB/c小鼠分为6组:空白时照组、DSS模型组、生理盐水(NS)组、柳氮磺胺吡啶(SASP)组、乳酸杆菌组和双歧杆菌组,10只/组,造模第8天处死小鼠,计算小鼠组织学损伤评分、评价组织病理学改变及结肠黏膜COX-2免疫组化情况.结果 乳酸杆菌组、双歧杆菌组及SASP组小鼠一般情况好,组织学损伤评分低,小鼠结肠黏膜COX-2的表达较DSS模型组明显减少.结论 乳酸杆菌和双歧杆菌可能通过抑制结肠黏膜COX-2的表达而减轻小鼠结肠黏膜的损伤,达到治疗UC的目的 .其疗效与SASP相当.

Effect of probiotcs on ulcerative colitis in mice

Objective To investigate the effect of COX-2 in the DSS-induced UC mouse model and the effects of probiotics in the pathogenesis of UC. Methods DSS-induced UC mouse model was used in the study. A total of 60 mice were divided into 6 groups (Blank control group, DSS model group, Saline group, SASP group, Lactovacillus group and Bifidobactefia group). After 8 days, all mice were sacrificed. Histological injury score and pathological change and COX-2 in mucous of colon membrane were evaluated. Results The stages and histological changes were obviously improved and the expression of COX-2 in the colonic mueosa of mouse with UC was decreased when using probiotics and SASP. Conclusions Lactobacillns and Bifidobacterla can decrease the expression of COX-2 in the colonic mucosa of mouse with UC. The effect has no difference between SASP and probiotic group.