Three-dimensional modeling of transport of nutrients for multicellular tumor spheroid culture in a microchannel |
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Authors: | Guoqing Hu Dongqing Li |
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Institution: | (1) Department of Mechanical Engineering, Vanderbilt University, VU Station B 351592, 2301 Vanderbilt Place, Nashville, TN 37235-1592, USA |
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Abstract: | The growth dynamics of avascular tumors in a microchannel bioreactor is investigated. A three-dimensional flow and nutrient
transport model, incorporating the multicellular tumor spheroid (MTS) growth model, has been developed to study the influence
of nutrients (oxygen and glucose) supply and distribution on the MTS growth. Numerical simulations based on the EMT6/Ro tumor
cells show that the continuous-flow perfusion is more efficient to deliver nutrients to the MTS than the diffusion-only static
culture. It is further demonstrated that as long as there is bulk flow, the growth of a single tumor spheroid at the early
stage is insensitive to the flow velocity and the channel size. For multiple tumor spheroids in the same microchannel, however,
increasing the perfusion velocity can improve the nutrient environment for the disadvantageous downstream tumor spheroid.
The flow shear stress exerting on the MTSs in the current microchannel bioreactor is estimated to be far below the critical
value to affect the MTS growth, which means that there is still much room for increasing perfusion velocity to satisfy the
higher nutrient requirement by the growing tumor spheroids. |
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Keywords: | Microchannel bioreactor Cell culture Multicellular tumor spheroid Numerical simulation |
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