慢性萎缩性胃炎大鼠胃黏膜细胞凋亡与中医证型的相关性

目的探讨大鼠不同证型慢性萎缩性胃炎(CAG)证候发生时胃黏膜细胞凋亡及调控蛋白表达水平的变化,从细胞凋亡的角度揭示CAG各证候的实质。方法复制大鼠脾虚证、肝郁证和湿热证CAG病证结合模型,检测胃黏膜上皮细胞凋亡水平和胃黏膜细胞凋亡调控蛋白Bcl-2、Fas抗原的表达水平。结果各模型组大鼠胃黏膜细胞凋亡指数均显著高于正常对照组,各证候模型组中以脾虚CAG组最高。各模型组大鼠胃黏膜Bcl-2、Fas蛋白表达量均显著高于正常对照组,各证候模型组中以脾虚CAG组最高。结论CAG大鼠胃黏膜细胞凋亡指数、Bcl-2、Fas蛋白表达与CAG证候有一定的相关性,以脾虚CAG组大鼠胃黏膜细胞凋亡指数最高和Bcl-2、Fas表达量最高。

Correlation between Apoptosis of Gastric Mucosa with Chinese Medical Pattern in Rats with Chronic Atrophic Gastritis

Objective To explore pattern essence from the aspect of apoptosis by observing apoptosis of gastric mucosa and expression of modulin in chronic atrophic gastritis (CAG) rats with different Chinese medical patterns. Methods CAG rat models with spleen deficiency pattern, liver depression pattern and damp heat pattern were copied respectively. Apoptosis of gastric mucosa and expression of Bcl-2 and Fas were detected. Results Apoptosis indexes of gastric mucosa and expression of Bcl-2 and Fas in model groups, especially in CAG with spleen deficiency group, were higher than those in normal control group. Conclusion There is correlation between apoptosis index of gastric mucosa and expression of Bcl-2 and Fas with Chinese medical pattern in CAG rats. Apoptosis index of gastric mucosa and expression of Bcl-2 and Fas is the highest in CAG with spleen deficiency group.