巨噬细胞对肌源性泡沫细胞生成的影响

平滑肌细胞增殖并形成泡沫细胞是动脉粥样硬化的中晚期病变特点之一。本实验研究了巨噬细胞对平滑肌细胞清道夫受体活性及肌源性泡沫细胞形成的影响。用细胞酶联免疫吸附法测定平滑肌细胞氧化型低密度脂蛋白的结合和摄入量以显示清道夫受活性，别测定细胞内胆固醇含量以显示脂质沉积。

Effects of Macrophages on Formation of Smooth Muscle Cell-derived Foam Cells

The smooth muscle cell-derived foam cell formation is one of the characteristics at the intermediate and late stages of atherosclerosis, In this paper we studied the effect of macrophages on the formation of smooth muscle cell-derived foam cells,The volumes of oxidized low density lipoprotein binding and uptake were measured with cell enzyme linked immunosorbent assay method to show the scavenger receptor activities,The contents of intracellular cholesterol were also measured.The results indicat ed that macrophage conditioned medium could induce the smooth muscle cells ( SMC ) scavenger receptor expression and its intracellular cholesterol accumulation,and that increase the SMC intracellular cholesterol contents, This supported that macrophages could release some factors to enhance the SMC scavenger receptor expression and the intracellular lipid accumulation, and that the interaction between SMC and macrophages may play an important role in the development of atherosclerosis.