紫珠属药用植物体外抗氧化作用

本实验测定了紫珠属药用植物批杷叶紫珠、紫珠及其果实、日本紫珠、大叶紫珠、华紫珠、老鸦糊抑制大鼠脏器体外孵育时自动发生脂质过氧化(LPO)50％的药物浓度(IC_(50)),发现除老鸦糊无抑制作用外,其余药物IC_(50)差别较大,以紫珠果实IC_(50)为最小,且IC_(50)与黄酮含量并不完全一致,进一步测定了枇杷叶紫珠、紫珠果实、紫珠对·OH引发的红细胞LPO及溶血抑制的IC_(50)、·OH引发的脑匀浆LPO抑制的IC_(50)、H_O_2与FeSO_4体系生成·OH的清除(或抑制)的IC_(50)发现IC_(50)大小顺序均为紫珠果实>紫珠>枇杷叶紫珠。结果表明,紫珠果实对体外自动发生LPO反应抑制最强,枇杷叶紫珠则对·OH引发的LPO反应抑制最强。

Effect of Callicarpa Herbs on Antioxidation in vitro

The concentration of half inhibition (IC50) of Callicarpa herbs, including Callicarpa kochiana(Ck), C. bodinieri (Cb) and its fruit (Cbf), C. japonica, C. macrophrlla, C. cathaarana, C. giraldi(Cg) on lipid peroxidation (LPO) of tissus homogenate incubated in 37 +/- 0.5 degrees C were tested. The result showed that all but Cg could inhibit lipid peroxidation. The IC50 of Cbf was minimum. IC50 of Cb, Cbf and Ck inhibiting red blood cell (RBC) LPO and hemolysis caused by hydroxyl free radical (.OH), inhibiting brain homogenate LPO induced by .OH and inhibiting or clean .OH formation by system of H2O2 and FeSO4 were also tested. It was found the IC50 were Cbf > Cb > Ck. These results suggested that the most strong inhibitor to automatic LPO reaction was Cbf, but to LPO caused by .OH was Ck.