人膜迷路三维精确建模与虚拟可视化

目的建立精确的人内耳膜迷路的三维模型，以满足内耳可视化研究和生物力学建模的需要。方法制作全系列、不染色、层厚20μm的新鲜成人颞骨火棉胶连续切片，使用幻灯机和高像素数码相机结合图像拼合技术获取全部切片的高清晰度数字图像。在3D-Doctor软件中，将全系列图像进行对位、设定标度参数、分割后，采用表面重建法生成内耳膜迷路三维模型。结果建立了能清晰显示膜迷路精细结构的内耳三维模型，在3D-Doctor软件中实现了不同的显示效果，并利用Cortona虚拟现实建模语言软件在个人电脑上实现了模型的桌面虚拟可视化。结论全系列不染色的火棉胶连续切片、数码相机拍摄结合图像拼合和高性能图像处理软件的使用可以满足膜迷路精确建模的需要，并能提高建模的精确度。利用虚拟现实软件和设备可以实现内耳膜迷路的虚拟可视化，有利于形态学研究和教学。

Precise three-dimensional modeling and virtual visualization of human membranous labyrinthin

Objective To establish a precise three-dimensional model of membranous labyrinth for further morphologic investigation and physiological research. Methods Complete series of serial unstained celloidin sections of a fresh human temporal bone were taken photos by high-pixel digital camera. The images were then processed with the technique of photo stitch and employed to reconstruct the three-dimensional model of the membranous labyrinth using the method of surface rendering. Results In 3D-Doctor software, the model was displayed by different methods. The model was also exported to VRML format and their virtual visualization was realized through the software of Cortona virtual reality modeling language viewer. Conclusions Precise modeling of membranous labyrinth could be realized by advanced imaging technique. With the advanced virtual reality software and equipments, the virtual visualization of membranous labyrinth could be realized, which would benefit the morphologic investigation and education.