金樱根、金樱茎多糖对小鼠肠道菌群失调的调整作用

目的:研究金樱根、金樱茎多糖对肠道菌群失调小鼠的调整作用。方法:用盐酸林可霉素15 g.kg-1ig,2次/d,连续3 d,建立小鼠肠道菌群失调模型,用含生药100 g.kg-1的金樱根、金樱茎多糖进行ig治疗,连续6 d,于第10天取新鲜粪便检测肠道正常菌群(肠杆菌、肠球菌、双歧杆菌和乳杆菌)的变化。CFU.g-1粪便=每一稀释度平均菌落数×稀释倍数/滴种体积(mL)。结果用1 g粪便中菌数的对数表示(lg10n.g-1)。同时设正常对照、阳性对照、模型对照及自然恢复组。结果:6 d治疗后,全樱根多糖组小鼠肠道主要菌群的数量基本恢复,其中肠球菌、肠杆菌、双歧杆菌优于自然恢复组(P<0.05);金樱茎多糖组仅乳杆菌数量高于模型组(P<0.01)。结论:金樱根、金樱茎多糖对正常小鼠肠道菌群失调有一定调整作用,且金樱根多糖效果优于金樱茎多糖。

Effect of Polysaccharides of Radix Rosa loevigata and Caulis Rosa laevigata on Intestinal Flora of Mouse

Objective:To research the effect of polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata on intestinal flora of mouse. Method: Intestinal microbe dysbiosis in mice were constructed by intragastric administration of lincomycin (15 g·kg^-1,bid, 3 d). The mice were given polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata (100 g·kg^-1 of crude drug for 6 days,) and then normal intestinal flora of fresh fecal was taken on the 10^th day to observe the changes in normal intestinal microflora in the first 10 days. The result was expressed as lg10ⁿ·g^-1.The nomal control, positive control and spontaneous recovery groups were set up. Result: Intestinal microbe dysbiosis in mice was successfully established. After the intestinal microbial flora in mice was disturbed and then treatment by polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata for 6 days, alteration of intestinal flora in polysaccharides of Radix Rosa laevigata was basically regulated. For polysaccharides of Caulis Rosae laevigatae, comparing with the modle groups, only the quantity of Enterobacteriaceae and Bifidobacterrium was increased significantly (P<0.01). Conclusion: The polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata have the function of regulating alteration of intestinal flora. Comparing with Caulis Rosae laevigatae, Radix Rosa laevigata has the better curative effect.