syndecan-4对碱性成纤维细胞生长因子诱导人肾小球系膜细胞增殖及细胞外基质分泌的影响

目的 研究syndecan-4对碱性成纤维细胞生长因子（bFGF）诱导人肾小球系膜细胞(HMC)增殖及细胞外基质（ECM）分泌的影响，并探讨syndecan-4-蛋白激酶Cα（PKCα）途径在其中的作用。 方法 免疫荧光方法观察syndecan-4在HMC上的表达。筛选有效的syndecan-4-siRNA转染HMC，噻唑蓝（MTT）比色法检测HMC在bFGF不同作用时间下的增殖差异；ELISA法检测细胞上清液中的Ⅰ、Ⅳ型胶原和纤连蛋白（FN）含量；荧光定量PCR观察syndecan-4和PKCα含量的变化。 结果　syndecan-4蛋白在HMC有表达。bFGF可促进HMC的增殖及FN、Ⅳ型胶原的分泌，使得每百万看家基因中PKCα拷贝数明显增加。转染syndecan-4 siRNA后，显著降低了HMC的增殖速度（48~60 h时点，P < 0.01）、ECM分泌（FN：24 h时点，P < 0.01，48~96 h，P < 0.05；Ⅳ型胶原：72~96 h时点，P < 0.05）及PKCα含量 （0 h时点，P < 0.05，12 ~48 h时点，P < 0.01）。 结论 syndecan-4参与调控bFGF诱导HMC的增殖及ECM分泌过程。syndecan-4-PKCα途径可能在其中扮演重要作用。

Effect of syndecan-4 on the proliferation and extracellular matrix secretion of human mesangial cells stimulated by basic fibroblast growth factor

Objective To investigate the effect of syndecan-4 on the proliferation and extracellular matrix (ECM) secretion of human mesangial cells(HMC) stimulated by basic fibroblast growth factor (bFGF) and to evaluate the role of syndecan-4-PKCα pathway. Methods The expression of syndecan-4 in HMC was observed by immunofluorescence. After the down-regulation of syndecan-4 in HMC by RNA interference, the cell proliferation was detected by MTT. The secretion of fibronectin (FIN), type IV collagen, type Ⅰ collagen was assessed by ELISA. The copy number of syndecan-4 and PKCα was measured by fluorescent quantitation PCR at different time points. Results Syndecan-4 was expressed in HMC. bFGF could promote the cell proliferation and ECM secretion together with the PKCα copy number per million house-keeping genes of HMC, which could be reversed by the syndecan-4 siRNA transfection (MtT: 48-60 h, P<0.01; FiN: 24 h, P<0.01, 48-96 h, P<0.05; type Ⅳ collagen: 72-96 h, P<0.05; PKCa: 0 h, P<0.05, 12-48 h, P< 0.01). Conclusion Syndecan-4 may regulate the proliferation and ECM secretion of HMC stimulated by bFGF through syndecan-4-PKCα pathway.