效应面法优化蒙花苷水解工艺及其水解物的醛糖还原酶抑制活性研究

目的：用效应面法对蒙花苷的水解工艺进行优化，通过醛糖还原酶模型研究其水解物金合欢素醛糖还原酶的抑制活性。方法：建立水解物金合欢素体外生物酶模型，通过测定NADPH的荧光吸收，计算水解物金合欢素对醛糖还原酶的抑制率，从而得到水解物的醛糖还原酶抑制活性的IC₅₀的平均值。在蒙花苷水解工艺中采用效应面法优化水解工艺，并对结果进行预测分析。结果：水解物金合欢素醛糖还原酶抑制活性，其IC₅₀的平均值为2.74 mg·L^-1。优选的水解工艺为水解时间7.4 h，硫酸甲醇0.54 mol·L^-1，固液比3：1。结论：水解物金合欢素具有比蒙花苷较好的醛糖还原酶抑制活性。优选的蒙花苷水解工艺简便，预测性良好。

Optimization of hydrolysis process of linarin using response surface methodology and research about ARI activity of glycosylation-acacetin

Objective: To optimize the hydrolysis process of linarin by response surface methodology, and to use the model of aldose reductase to study the acacetin's activity of aldose reductase inhibitory. Method: The model of acacetin enzyme in vitro was established by the determination of fluorescence absorption of NADPH, the inhibition rate of acacetin aldose reductase was calculated, and then the IC₅₀ of hydrolysis was obtained. The hydrolysis process of linarin hydrolysis condition was optimized by using response surface method. Result: The results indicated that the IC₅₀ of acacetin (2.74 mg·L^-1) was less than the IC₅₀ of linarin (3.53 mg·L^-1). Hydrolyzation time of 7.4 h, sulphuric acid concentration of 0.54 mol·L^-1 and the ratio of material to liquid of 3:1 were the optimum conditions. Conclusion: Hydrolyzate acacetin has preferable inhibitory activity of aldose reductase. The optimized hydrolysis condition of linarin is convenient to use with good predictability.