| UPLC法测定倍他米松尿素乳膏中倍他米松含量 |
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| 引用本文： | 张振兴,孙旭,高媛,陈瑶,刘云云,周平,黄国海.UPLC法测定倍他米松尿素乳膏中倍他米松含量[J].中国药师,2014(7):1116-1118. |
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| 作者姓名： | 张振兴 孙旭 高媛 陈瑶 刘云云 周平 黄国海 |
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| 作者单位： | 济南军区联勤部药品仪器检验所 济南 250022；;济南军区联勤部药品仪器检验所 济南 250022；;济南军区联勤部药品仪器检验所 济南 250022；;济南军区联勤部药品仪器检验所 济南 250022；;济南军区联勤部药品仪器检验所 济南 250022；;解放军第155中心医院；;解放军第二炮兵96546部队保障部 |
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| 基金项目： | 济南军区后勤科研计划（编号：CJN12L078） |
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| 摘 要： | 目的：建立倍他米松尿素乳膏含量测定方法.方法：色谱柱：Agilent ZORBAX SB-C18（250 mm×4.6mm,5μm）,流动相为乙腈-水（30∶70）,流速1.0 ml·min-1,检测波长240 nm,柱温45℃,进样量20 μl.结果：倍他米松在12.35～92.64 μg·ml-1的范围内与峰面积呈良好线性关系（r =1.000 0）,平均回收率为98.02％,RSD为0.74％（n=9）.结论：建立的方法结果准确、稳定性好、专属性强,可用于倍他米松尿素乳膏中倍他米松含量测定.
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| 关 键 词： | 超高效液相色谱法 倍他米松尿素乳膏 倍他米松 含量测定 |
| 收稿时间： | 2014/2/25 0:00:00 |
| 修稿时间： | 4/4/2014 12:00:00 AM |
Determination of Betamethasone in Betamethasone Urea Creams by UPLC |
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| Zhang Zhenxing,Sun Xu,Gao Yuan,Chen Yao,Liu Yunyun,Zhou Ping and Huang Guohai.Determination of Betamethasone in Betamethasone Urea Creams by UPLC[J].China Pharmacist,2014(7):1116-1118. |
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| Authors: | Zhang Zhenxing Sun Xu Gao Yuan Chen Yao Liu Yunyun Zhou Ping and Huang Guohai |
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| Institution: | Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China;;Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China;;Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China;;Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China;;Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China;;The 155th Central Hospital of PLA;;Joint Logistics Department of Jinan Military Area Drug and Instrument Control, Jinan 250022, China; 2.The 155th Central Hospital of PLA; 3.Security Departemtn, PLA Second Artillery Unit 96546 |
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| Abstract: | Objective：To establish a method for the determination of betamethasone urea creams.Methods：An Agilent ZORBAX SB-C18 （250 mm × 4.6 mm,5 μm） column was used,the mobile phase was acetonitrile-water（30∶ 70）,the flow rate was 1.0 ml · min-1,the detection wavelength was 240nm,the column temperature was 45℃ and the injection volume was 20 μl.Results：Betamethasone concentration had the linear relationship with the peak area.within the range of 12.35-92.64 μg · ml-1（r =1.000 0）,and the average recovery was 98.02％ （RSD =0.74％,n =9）.Conclusion：The method is accurate,stable and specific,and can be used to determine betamethasone in betamethasone urea creams. |
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| Keywords: | Ultra performance liquid chromatography Betamethasone urea creams Betamethasone Determination |
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