| MicroRNA-34a对尿路上皮癌细胞株5637细胞迁移、侵袭和增殖的影响 |
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| 引用本文： | 张颖,张超.MicroRNA-34a对尿路上皮癌细胞株5637细胞迁移、侵袭和增殖的影响[J].海南医学,2013,24(22):3277-3280. |
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| 作者姓名： | 张颖 张超 |
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| 作者单位： | 张颖(深圳市龙华新区人民医院手术室,广东 深圳,518100)； 张超 (天津市肿瘤医院泌尿肿瘤科,天津,300060)； |
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| 基金项目： | 天津医科大学附属肿瘤医院引进人才与博士启动基金(项目编号：B1208) |
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| 摘 要： | 目的 研究microRNA-34a （miR-34a）对膀胱肿瘤细胞株5637细胞迁移、侵袭和增殖的影响.方法 在5637细胞株中过表达miR-34a,运用小室迁移实验和小室侵袭实验检测细胞迁移力和侵袭力;运用血球计数板法和新型四唑氮盐（MTS）法检测细胞增殖力.结果 过表达miR-34a后,5637细胞迁移力和侵袭力均下降（P＜0.01）,细胞增殖受抑（P＜0.01）.结论 过表达miR-34能抑制膀胱肿瘤细胞株5637的迁移、侵袭和增殖.
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| 关 键 词： | 尿路上皮癌 迁移 侵袭 增殖 microRNA-34a |
Effects of MicroRNA-34a on the cell migration,invasion and proliferation of bladder tumor cell line 5637 |
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| ZHANG Ying,ZHANG Chao.Effects of MicroRNA-34a on the cell migration,invasion and proliferation of bladder tumor cell line 5637[J].Hainan Medical Journal,2013,24(22):3277-3280. |
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| Authors: | ZHANG Ying ZHANG Chao |
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| Institution: | 1. Operating Room, People ＂s Hospital of Longhua New District of Shenzhen, Shertzhen 518100, Guangdong, CHINA; 2. Department of Urology ＆ Oneology, Tianjin Cancer Hospital, Tianjin 300060, CHINA) |
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| Abstract: | Objective To evaluate the effects of microRNA-34a （miRNA-34a） on cell migration,invasion and proliferation of bladder cancer cell line 5637.Methods miR-34a was overexpressed in bladder cancer cell line 5637.Transwell migration assay and transwell invasion assay were performed to predict cell migration ability and invasion ability,respectively.Cell proliferation was assessed by cell counting assay and MTS assay.Results The overexpression of MiR-34a significantly down-regulated cell migration and cell invasion of bladder cancer cell line 5637 （P〈0.01）,and significantly decreased cell proliferation （P〈0.01）.Conclusion The overexpression of microRNA-34a inhibits cell migration,invasion and proliferation in bladder cancer cell line 5637. |
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| Keywords: | Urothelial carcinoma Migration Invasion Proliferation microRNA-34a |
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