| 角质形成细胞和黑素细胞体外共培养的实验研究 |
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| 引用本文： | 龚石,张晴. 角质形成细胞和黑素细胞体外共培养的实验研究[J]. 海南医学, 2013, 24(21): 3128-3130 |
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| 作者姓名： | 龚石 张晴 |
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| 作者单位： | 龚石 (海南省皮肤病医院皮肤科,海南海口,570206); 张晴 (贵阳医学院药理教研室,贵州 贵阳,550004); |
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| 摘 要： | 目的 利用两种不同培养方案,探讨正常人角质形成细胞和黑素细胞的体外共同培养方法.方法 采用无血清培养液和微量血清培养液分别进行人角质形成细胞和黑素细胞纯化培养及用K-SFM角质形成细胞培养基开展两种细胞共同培养.结果 K-SFM角质形成细胞培养基及其添加成分构成的无血清培养基成功进行角质形成细胞和黑素细胞共同培养.结论 不同培养方案可产生不同体外共同培养结果,可以成功在体外进行角质形成细胞-黑素细胞共同培养.
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| 关 键 词： | 角质形成细胞 黑素细胞 共同培养 |
Co-culture of melanocyte-keratinocyte in vitro |
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| GONG Shi,ZHANG Qing. Co-culture of melanocyte-keratinocyte in vitro[J]. Hainan Medical Journal, 2013, 24(21): 3128-3130 |
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| Authors: | GONG Shi ZHANG Qing |
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| Affiliation: | 1. Department of Dermatology, HainanSkin Disease Hospital, Haikou 570206, Hainan, CHINA; 2. Department of Pharmacology, Guiyang Medical College, Guiyang 550004, Guizhou, CHINA) |
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| Abstract: | Objective To study melanocyte-keratinocyte co-culture in vitro by two different methods. Methods Keratinocytes and melanocytes were cultured from free serum medium, and coculture was performed ap- plying K-SFM frec serum medium. Results In K-SFM free serum medium, keratinocytes and melanocytes was co-cultured successfully. Conclusion Different cell culture medium designs give rise to different results, which can all successfully culture keratinocyte-melanocyte in vitro. |
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| Keywords: | Keratinocvtes： Melanocvtes： Co-culture |
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