Examination of endothelial cell‐induced epidermal regeneration in a mice‐based chimney wound model |
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Authors: | Joseph Seo MS Soon‐Jung Park MS Jong‐Jin Choi BS Sun‐Woong Kang PhD Joa‐Jin Lim PhD Hye‐Jin Lee MS Jong‐Soo Kim PhD Heung‐Mo Yang PhD Sung‐Joo Kim MD PhD Eun‐Young Kim PhD Se‐Pil Park PhD Sung‐Hwan Moon PhD Hyung‐Min Chung PhD |
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Affiliation: | 1. Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Korea;2. Human and Environmental Toxicology Program, University of Science and Technology, Daejeon, Korea;3. Stem Cell Research Laboratory, CHA Stem Cell Institute, CHA University, Seoul, Korea;4. Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea;5. Mirae Cell Bio Inc./Jeju National University Stem Cell Research Center, Seoul, Korea;6. Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, Korea;7. Department of Medicine, School of Medicine, Konkuk University, Seoul, Korea |
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Abstract: | As wound contraction in the cutaneous layer occurs rapidly in mice, mechanical means are typically used to deliberately expose the wound to properly investigate healing by secondary intention. Previously, silicon rings and splinting models were attempted to analyze histological recovery but prevention of surrounding epidermal cell migration and subsequent closure was minimal. Here, we developed an ideal chimney wound model to evaluate epidermal regeneration in murine under hESC‐EC transplantation through histological analysis encompassing the three phases of regeneration: migration, proliferation, and remodeling. Human embryonic stem cell derived endothelial cells (hESC‐EC) were transplanted due to possessing a well‐known therapeutic effect in angiogenesis which also enhances epidermal repair to depict the process of regeneration. Following a standard 1 mm biopsy punch, a chimney manufactured by modifying a 1.7 mL microtube was simply inserted into the excisional wound to complete the modeling process. Under this model, the excisional wound remained fully exposed for 14 days and even after 4 weeks, only a thin transparent layer of epidermal tissue covered the wound site. This approach is able to more accurately depict epidermal repair in relation to histology while also being a user‐friendly and cost‐effective way to mimic human recovery in rodents and evaluate epithelial repair induced by a form of therapy. |
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