| 毛萼乙素抑制人正常及肝癌血管生成的作用研究 |
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| 引用本文： | 班立丽,张慧,罗敏,杨兵兵,韩宏升,潘敏,李玛琳.毛萼乙素抑制人正常及肝癌血管生成的作用研究[J].中国药房,2010(15):1351-1353. |
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| 作者姓名： | 班立丽 张慧 罗敏 杨兵兵 韩宏升 潘敏 李玛琳 |
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| 作者单位： | 昆明医学院第二附属医院药剂科;昆明医学院省天然药物药理重点实验室; |
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| 摘 要： | 目的:研究毛萼乙素对人脐静脉内皮细胞(HUVEC)增殖的影响以及对人肝癌血管生成的影响。方法:采用改良MTT法测试6个浓度的毛萼乙素在体外对原代培养的HUVEC增殖的影响;用人肝癌鸡胚尿囊膜血管复制模型,测定毛萼乙素在器官水平对血管生成的抑制作用。结果:毛萼乙素对HUVEC增殖具有显著的抑制作用,半数抑制浓度(IC50)=7.27μg·mL-1;抑制效应呈剂量和时间依赖性。在剂量为25、50、100μg·mL-1时对人肝癌鸡胚尿囊膜血管面积比的抑制率分别为14.15%、48.72%、60.63%,抑制作用中等;其中剂量在50μg·mL-1时与阳性对照反应停相当(P>0.05)。结论:毛萼乙素对人脐静脉内皮细胞的增殖有显著的抑制作用,并能显著抑制人肝癌细胞诱导的鸡胚尿囊膜血管生成。
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| 关 键 词： | 毛萼乙素 人脐静脉内皮细胞 血管生成 肝癌细胞 鸡胚尿囊膜 |
Inhibition Effect of Eriocalyxin B against Angiogenesis of Hepatocelluar Carcinoma and Human Normal Endothelial Cell |
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| BAN Li-li ZHANG Hui,LUO Min,YANG Bing-bing,HAN Hong-sheng,PAN Min,LI Ma-lin.Inhibition Effect of Eriocalyxin B against Angiogenesis of Hepatocelluar Carcinoma and Human Normal Endothelial Cell[J].China Pharmacy,2010(15):1351-1353. |
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| Authors: | BAN Li-li ZHANG Hui LUO Min YANG Bing-bing HAN Hong-sheng PAN Min LI Ma-lin |
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| Institution: | BAN Li-li(Dept. of Pharmacy,The Second Affiliated Hospital of Kunming Medical College,Kunming 650101,China) ZHANG Hui,LUO Min,YANG Bing-bing,HAN Hong-sheng,PAN Min,LI Ma-lin(Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical College,Kunming 650031,China) |
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| Abstract: | OBJECTIVE:To study the effect of eriocalyxin B (ER-B) on the proliferation of human umbilical vein endothelial cell (HUVEC) and hepatocelluar carcinoma angiogenesis. METHODS:The effects of ER-B at 6 kinds of concentration on the proliferation of HUVEC cultured in vitro were detected using modified MTT assay. Human hepatic cancer chicken chorioallantoic membrane angiogenesis model was induced to determine the inhibition effect of ER-B against angiogenesis. RESULTS:ER-B showed significant inhibitory effects o... |
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| Keywords: | Eriocalyxin B Human umbilical vein endothelial cell Angiogenesis Liver cancer cell Chicken chorioallantoic membrane |
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