203例汉族人群9个STR基因座的遗传多态性分析

目的:分析中国汉族无关个体的9个STR基因座的多态性,研究其在法医鉴定中的应用价值。方法:应用STR_Typer_10_vl荧光标记试剂盒,对203例中国汉族无关个体的9个基因座D18S1364、D12S391、D13S325、D6S1043、D2S1772、D11S2368、D22-GATA198B05、D8S1132和D7S3048进行复合扩增,用310遗传分析仪和Gene Mapper IDv3.2对扩增产物进行分型,计算各个基因座的等位基因频率,统计其遗传学参数。结果:9个STR基因座共检出105个等位基因、367种基因型。各个基因座的等位基因数在9～17个之间,基因频率在0.003～0.277之间,杂合度在0.763～0.865之间,三联体非父排除率在0.533～0.726之间,个人识别率在0.910～0.965之间。9个STR基因座的累积个人识别率为0.999 999 999 998 122 1,三联体累积非父排除率为0.999 955 67。经H-W平衡检验,9个STR基因座的P值均>0.05。结论:该9个STR基因座在中国汉族人群中均属于多态性程度高的遗传标记,可以用于法医亲权鉴定和个人识别,也可以用于人类学和遗传学研究。

A study on genetic polymorphisms of nine short tandem repeat loci in Chinese Han population

Objective： To investigate genetic polymorphism of 9 short tandem repeat （STR） loci of 203 unrelated individuals in Han population and assess their usefulness in forensic casework. Methods： Nine loci （D18S1364, D12S391, D13S325, D6S1043, D2S1772, D11 $2368, D22 - GATA198B05, D8S1132 and D7S3048） of 203 DNA samples were amplified using STR Typer_10_vl kit, and the PCR products were analyzed and genotyped by ABI 310 genetic analyzer. Results： One hun- dred and five alleles and 367 genotypes were observed. The number of alleles was between 9 and 17. The allele frequencies and heterozygote were 0. 003 to 0. 277 and 0. 763 to 0. 865, respectively. Power of discrimination was between 0. 910 and 0. 965. The cumulated power of discriminating was 0.999 999 999 998 122 1, and the cumulative chance of exclusion of triplet was 0.999 955 67. These 9 loci were in accord with Hardy - Weinberg equilibrium. Conclusion： The multiplex amplification system of 9 loci is a useful tool in forensic paternity test, genetic and anthropological study.