| 克矽平对矽肺组织胶原基因表达的抑制作用 |
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| 引用本文： | 何玉先,缪庆.克矽平对矽肺组织胶原基因表达的抑制作用[J].中国药学杂志,1995,30(5):271-273. |
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| 作者姓名： | 何玉先 缪庆 |
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| 作者单位： | 1.北京地坛医院研究室；中国预防医学科学院劳动卫生与职业病研究所 |
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| 摘 要： | 使用Ｐｒｏａ＿１（Ⅰ），Ｐｒｏａ＿１（Ⅲ）人胶原ｃＤＮＡ探针，采用ｃＤＮＡ－ｍＲＮＡ斑点杂交技术，观察了染石英尘２个月、４个月矽肺大鼠及克矽平（ＰＶＮＯ）治疗１个月、３个月后肺组织中Ⅰ型和Ⅲ型前胶原ｍＲＮＡ水平的改变情况。结果表明，矽肺组织中Ⅰ、Ⅲ型前胶原ｍＲＮＡ含量比正常肺组织明显增加（Ｐ＜０．０５）；经克矽平治疗后两型胶原ｍＲＮＡ皆显著减少。我们认为矽肺病变中胶原蛋白的积聚是由石英粉尘引起胶原基因表达改变所致。药物ＰＶＮＯ能直接或间接地抑制胶原基因的转录，从而抑制矽肺病变时胶原蛋自的合成。
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| 关 键 词： | 矽肺 胶原基因 |
| 收稿时间： | 1994-01-24; |
Effects of polyvinylpyridine oxide on gene expression of type Ⅰ andⅢcollagens in experimental silicosis |
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| He Yuxian,Miao Qing,Liu Bingci.Effects of polyvinylpyridine oxide on gene expression of type Ⅰ andⅢcollagens in experimental silicosis[J].Chinese Pharmaceutical Journal,1995,30(5):271-273. |
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| Authors: | He Yuxian Miao Qing Liu Bingci |
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| Institution: | 1.Research Department of Beijing Ditan Hospital,Beijing 100011；Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing100050 |
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| Abstract: | Human Pro a_1(Ⅰ),Pro a_1(Ⅲ)collagens cDNA probes and RNA dot blot were em-ployed to determine the levels of collagen mRNA in the lung of silicotic rats treated bypolyvinylpyridine oxide(PVNO). The results showed that Pro a_1(Ⅰ) ,Pro a_1(Ⅲ) , mRNA Ievelsincreased significantly at 60 and 120 days after the rats were exposed to silica dust. The mRNAlevels went down at 1 and 3 months after PVNO treatmant. The results suggest that PVNO mayinhibit the gene expression of lung collagen in silicosis. |
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| Keywords: | silicosis collagen gene polyvinylpyridine oxide |
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