趋化因子CCL5在非小细胞肺癌中的表达及生物学功能探究

目的 探讨CC基序趋化因子配体5(CCL5)在非小细胞肺癌(NSCLC)中的表达情况及临床应用价值。方法 收集在徐州医科大学附属宿迁医院首次就诊确诊为NSCLC并行手术治疗的72例患者癌组织、癌旁组织及其临床资料，用免疫组化法检测癌组织及癌旁组织CCL5表达情况；用Realtime PCR检测CCL5mRNA在NSCLC和健康对照者血清中的表达；分析CCL5表达情况与患者临床、病理特征之间的相关性及其对预后影响。体外培养肺腺癌A549细胞，siRNA-CCL5转染细胞，观察下调CCL5表达后细胞增殖、迁移、侵袭能力的变化。结果 TCGA数据分析结果显示CCL5mRNA表达水平在肺腺癌和鳞癌中明显增加；免疫组化结果显示CCL5在NSCLC组织表达较癌旁组织明显上调，并且CCL5高表达与NSCLC患者淋巴结转移情况、TNM分期存在正相关(P=0.019,P=0.033);而与患者性别、年龄、病理类型无明显相关性；NSCLC血清中高表达CCL5mRNA,K-M分析结果显示血清CCL5 mRNA表达的上调与患者无进展生存率(PFS)存在明显的相关性；Cox多因素比例风险模型评估提示组织中CCL...

Expression and clinical significance of CCL5 in non small cell lung cancer

To detect the expression of CCL5 in tissue and serum of patients with NSCLC and explore the clinical significance of expression of CCL5. Methods Cancer tissues, adjacent tissues and clinical data of 72 patients with NSCLC who were diagnosed for the first time and underwent surgical treatment in Suqian Hospital of Xuzhou Medical University were collected. Immunohistochemistry was used to detect the expression of CCL5 in cancer and adjacent tissues. Realtime PCR was used to detect the expression of CCL5mRNA in serum of NSCLC and healthy controls. The correlation between CCL5 expression and clinical and pathological features of patients and its influence on prognosis were analyzed. The lung adenocarcinoma A549 cells, siRNA CCL5 transfection cells were cultivated In vitro. The changes of cell proliferation, migration and invasion after down-regulating CCL5 expression were observed. Results The expression of CCL5 in tissue and serum of NSCLC patients was high, CCL5 expression was correlated with the degree of NSCLC differentiation and TNM stage (P=0.019, P=0.033). The K-M analysis results showed that the upregulation of CCL5 expression was significantly correlated with the decrease of overall survival rate. Silencing CCL5 expression can inhibit the proliferation and invasion of lung adenocarcinoma. Conclusion CCL5 expression is elevated in NSCLC and may contribute to the invasion and metastasis of lung cancer, suggesting that CCL5 might be a prognostic factor in lung adenocarcinoma.