| HPLC法同时测定仙人菇口服液中10种成分的含量 |
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| 引用本文： | 高守红,庞涛,江道振,王志鹏,陆文铨,岳小强,赵婧.HPLC法同时测定仙人菇口服液中10种成分的含量[J].第二军医大学学报,2023,44(9). |
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| 作者姓名： | 高守红 庞涛 江道振 王志鹏 陆文铨 岳小强 赵婧 |
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| 作者单位： | 海军军医大学第二附属医院药学部,海军军医大学第二附属医院药学部;海军军医大学第二附属医院中医科,海军军医大学第二附属医院,海军军医大学第二附属医院药学部,海军军医大学第二附属医院药学部,海军军医大学第二附属医院中医科,海军军医大学第二附属医院中医科 |
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| 基金项目： | ]上海市科学技术委员会( NO: 21S21902700 )． Foundation Project of Shanghai Science and Technology Commission ( NO: 21S21902700 )[共同第一作者] △[作者介绍] 高守红,女,硕士,副主任药师,E-mail:gaoshouhong@126.com；庞涛(1984-),男,硕士,主管药师,研究方向：临床药学。E-mail: schwieni_tao@163.com；*通讯作者(Corresponding authors):赵婧,女,上海,中医结合临床博士,副主任医师,副教授,Tel:021-81885074, E-mail:tozj@163.com △,江道振2,王志鹏1,陆文铨1,岳小强3,赵婧3* |
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| 摘 要： | 目的：建立快速、准确的高效液相色谱法(HPLC)同时测定仙人菇口服液中腺苷、虫草素、芦丁、芒柄花苷、朝藿定B、朝藿定C、淫羊藿苷、毛蕊异黄酮、灵芝酸A、宝藿苷I等10种有效成分的含量。方法：使用Dikma Diamonsil Plus C18柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%冰醋酸水溶液梯度洗脱；流速为1.0 ml/min,柱温为30°C,检测波长为270 nm,进样量为10 μl。结果：腺苷在0.25~16.0 μg/ml、虫草素和淫羊藿苷在6.25~400.0 μg/ml、芦丁在1.25~80.0 μg/ml、芒柄花苷在0.5~32.0 μg/ml、朝藿定B在0.375~24.0 μg/ml、朝藿定C在3.125~200.0 μg/ml、毛蕊异黄酮和灵芝酸A在0.625~40.0 μg/ml、宝藿苷I在0.125~8.0 μg/ml浓度范围内呈良好的线性关系(r>0.999)；精密度、稳定性、重复性实验的RSD值均小于5%；加样回收率在85%~115%范围内。结论：该法操作简单、准确性高、重复性好,适用于仙人菇口服液中腺苷、虫草素、芦丁、芒柄花苷、朝藿定B、朝藿定C、淫羊藿苷、毛蕊异黄酮、灵芝酸A、宝藿苷I等10种有效成分的含量测定,可以作为仙人菇口服液质量控制方法。
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| 关 键 词： | HPLC 仙人菇口服液 质量控制 含量测定 |
| 收稿时间： | 2021/8/24 0:00:00 |
| 修稿时间： | 2023/6/7 0:00:00 |
Simultaneous Determination of 10 Constituents in Xianrengu Oral Liquild by HPLC |
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| GAO Shou-Hong,PANG Tao,JIANG Dao-zhen,WANG Zhi-peng,LU Wen-Quan,YUE Xiao-Qiang and ZHAO Jing.Simultaneous Determination of 10 Constituents in Xianrengu Oral Liquild by HPLC[J].Academic Journal of Second Military Medical University,2023,44(9). |
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| Authors: | GAO Shou-Hong PANG Tao JIANG Dao-zhen WANG Zhi-peng LU Wen-Quan YUE Xiao-Qiang and ZHAO Jing |
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| Institution: | Department of Pharmacy,Second Affiliated Hospital of Naval Medical University,Department of Pharmacy,Second Affiliated Hospital of Naval Medical University;China Department of Thyroid Breast and Hernia Surgery,Second Affiliated Hospital of Naval Medical University;China;Department of Traditional Chinese Medicine,Second Affiliated Hospital of Naval Medical University;China,Department of Thyroid Breast and Hernia Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China,Department of Pharmacy,Second Affiliated Hospital of Naval Medical University,Department of Pharmacy,Second Affiliated Hospital of Naval Medical University,Department of Traditional Chinese Medicine,Second Affiliated Hospital of Naval Medical University,Department of Traditional Chinese Medicine,Second Affiliated Hospital of Naval Medical University |
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| Abstract: | |
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| Keywords: | HPLC Xianrengu Oral Liquid Quality Control Content Determination |
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