GLP-1RA艾塞那肽调控骨骼代谢的作用研究

目的 为了探讨胰高血糖素样肽-1 (glucagon-like peptide-1,GLP-1)受体激动剂对糖尿病患者的成骨细胞和破骨细胞的影响。方法 利用小鼠成骨细胞MC3T3-E1和小鼠单核巨噬细胞RAW264.7模拟成骨细胞和破骨细胞。在高糖培养基中分别加入不同浓度的艾塞那肽孵育72 h,利用RT-qPCR技术分别检测成骨细胞MC3T3-E1中GLP1-R、ALP、LRP-5和破骨细胞RAW 264.7中GLP1-R、Cathespin K的表达情况。结果 艾塞那肽诱导成骨细胞MC3T3-E1细胞中GLP-1R、ALP、LRP-5基因的表达显著升高，而明显抑制破骨细胞RAW 264.7中GLP1-R、Cathespin K的表达。结论 GLP-1受体激动剂艾塞那肽可能通过影响成骨细胞及破骨细胞的分化，从而对糖尿病引起的骨质疏松起到一定程度的改善。

Effects of GLP-1RA Exenatide-4 on bone metabolism

Objective To explore the effect of glucagon-like peptide-1(GLP-1) receptor agonist on osteoblast and osteoclast induced by diabetes mellitus. Methods Murine osteoblast MC3T3-E1 and mononuclear macrophage RAW264.7 were treated to simulate osteoblasts and osteoclasts. Following a 72h different concentrations of?Exenatide-4 incubation in high glucose medium, RT-qPCR was used for detecting the expressions of GLP1-R, ALP and LRP-5 in osteoblast MC3T3-E1 and GlP1-R, Cathespin K in osteoclast RAW 264.7. Results Exenatide-4 significantly increased the expression of GLP-1R, ALP and LRP-5 in MC3T3-E1 while dramatically decreasing GLP1-R and Cathespin K in RAW 264.7. Conclusion Exenatide-4, a GLP-1 receptor agonist, may reduce diabetes-related osteoporosis through affecting the differentiation of osteoblasts and osteoclasts.