| 通络救脑注射液对成年大鼠侧脑室室管膜下层Vimentin、GFAP阳性细胞的影响 |
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| 引用本文： | 司银楚,李兴刚,李海燕,李光媛,沈钦华,商志伟.通络救脑注射液对成年大鼠侧脑室室管膜下层Vimentin、GFAP阳性细胞的影响[J].中华中医药杂志,2009(6). |
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| 作者姓名： | 司银楚 李兴刚 李海燕 李光媛 沈钦华 商志伟 |
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| 作者单位： | 北京中医药大学基础医学院;湖南省张家界市中医院; |
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| 基金项目： | “十一五”国家科技支撑计划项目(No.2007BAI07A23) |
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| 摘 要： | 目的:观察通络救脑注射液对成年大鼠侧脑室室管膜下层Vimentin、胶质原纤维酸性蛋白(GFAP)阳性细胞的作用。方法:采用Wistar成年雄性大鼠,去除大鼠左侧大脑皮层血管,应用免疫组化方法观察去皮层血管对侧脑室室管膜下区(SVZ)神经干细胞增殖、分化和迁移的影响,以及通络救脑注射液的作用。结果:通过连续切片的免疫组化结果显示去皮层血管后侧脑室SVZ的增殖细胞核抗原(PCNA)、Vimentin、GFAP阳性细胞均明显增多,反应增强,表象改变显著。此种损伤刺激引起了相对静止的SVZ神经干细胞增殖、分化,给予通络救脑注射液后促进以上各种阳性细胞表达增多、增强。结论:提示通络救脑注射液可能通过上调内源性促神经生长因子的表达促进去皮层血管后成年大鼠SVZ神经干细胞增殖、分化。
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| 关 键 词： | 大鼠 室管膜下细胞 通络救脑注射液 实验研究 |
Effects of cortical devascularization on Vimentin,GFAP positive cells located on SVZ of adult rat and the role of Tongluojiunao Injection |
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| SI Yin-chu,LI Xing-gang,LI Hai-yan,LI Guang-yuan,SHEN Qin-hua,SHANG Zhi-wei.Effects of cortical devascularization on Vimentin,GFAP positive cells located on SVZ of adult rat and the role of Tongluojiunao Injection[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(6). |
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| Authors: | SI Yin-chu LI Xing-gang LI Hai-yan LI Guang-yuan SHEN Qin-hua SHANG Zhi-wei |
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| Institution: | SI Yin-chu1,LI Xing-gang2,LI Hai-yan1,LI Guang-yuan1,SHEN Qin-hua1,SHANG Zhi-wei1(1Preclinical Medicine College,Beijing University of Chinese Medicine,Beijing 100029,China,2Hospital of Chinese Medicine of Zhangjiajie of Hunan Province,Zhangjiajie 427100,China) |
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| Abstract: | Objective:To study the effects of Tongluojiunao Injection on the Vimentin,GFAP positive cells located on the SVZ of adult rat.Methods:In this study,we use adult male Wistar rats,remove the left cortex vessels and adopt the immunohistochemistry(IHC) methods to explore the effects of devascularization of cortex on the proliferation,differentiation and migration of the SVZ NSCs,and in the meantime,the role of the Tongluojiunao Injection.Results:Through the IHC results of serial sections,we see that after the d... |
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| Keywords: | Rat Subventricular cells Tongluojiunao Injection Experiment research |
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