Nicorandil opens mitochondrial KATPchannels not only directly but also througha NO-PKG-dependent pathway

Nicorandil, a hybrid of nitrate generator and potassium channel opener, protects ischemic myocardium by opening mitochondrial ATP sensitive potassium (mitoK_ATP) channels. We recently found that nitric oxide (NO) opened K_ATP channels in rabbit hearts by a protein kinase G (PKG) mechanism. This study examined whether the NO-donor property of nicorandil also contributes to opening of mitoK_ATP channels through PKG. MitoK_ATP channel opening was monitored in adult rabbit cardiomyocytes by measuring reactive oxygen species (ROS) production, an established marker of channel opening. Nicorandil increased ROS production in a dose-dependent manner. The selective mitoK_ATP channel inhibitor 5-hydroxydecanoate (200 μM) completely blocked ROS production by nicorandil at all doses. The PKG inhibitor 8-bromoguanosine-3’,5’-cyclic monophosphorothioate, Rpisomer (Rp-8-Br-cGMPs, 50 μM) shifted the dose-ROS production curve to the right with an increase of the EC₅₀ from 2.4 x 10^–5 M to 6.9 x 10^–5 M. Rp- 8-Br-cGMPs did not affect the increase in ROS production by the selective mitoK_ATP channel opener diazoxide while it completely blocked increased ROS production from the NO donor S-nitroso-N-acetylpenicillamine (1 μM). Furthermore ODQ, an antagonist of soluble guanylyl cyclase, blocked nicorandil’s ability to increase ROS generation. These results indicate that nicorandil, in addition to its direct effect on the channels, opens mitoK_ATP channels indirectly via a NO-PKG signaling pathway.