少量人类精子以空卵透明带为冻贮载体的冷冻保存

目的:探讨空卵透明带作贮存载体冷冻保存少量人类精子。方法:将人或金黄地鼠卵内的细胞成份全部除去,制备成空卵透明带,分别显微注入人睾丸精子、附睾精子和射出精子后冷冻保存,并与正常供精者射出精子作对照。结果:解冻后卵透明带在溶液中容易识别寻找,卵透明带内的精子易于观察。附睾精子组(n=11)与供精者射出精子组(n=7)的冷冻精子活动率和存活率无显著性差异(P>0.05),但睾丸精子组(n=7)这两项参数值均显著低于附睾精子和射出精子组(P<0.01)。含6％、7.5％和9％不同甘油浓度的冷冻保护剂,对冷冻精子活动率无显著性影响(P>0.05);冻贮于人和金黄地鼠空卵透明带内的精子,两者的冷冻精子活动率也无显著性差异(P>0.05〕。结论:空卵透明带是冻贮少量人类精子的合适载体。

Cryopreservation of a Small Number of Human Sperm within Empty Zona Pellucidae

Objective: To investigate the empty zona pellucida for use in the cryopreservation of human sperm. Materials & Methods:Human and hamster zona pellucidae were evacuated and injected with testicular, epi-didymal and ejaculated sperm respectively. The zona pellucidae with sperm were cryopreserved. Results: After thawing, zona pellucidae were easily found, and sperm inside zona pellucidae were also easily observed. There were no differences in post-thaw motility and vitality between ejaculated and epididymal sperm groups (P>0. 05), but these two parameters were lowered in testicular sperm group compared to both ejaculated and epididymal sperm (P<0. 01). No significant difference was observed in post-thaw motilities among 6%, 7. 5%, 9% glycerol concentrations (P>0. 05). In addition, obvious differences in post-thaw motilities were not found between human and hamster empty zona pellucidae (P>0. 05). Conclusion:An evacuated zona pellucida is an ideal vehicle for the cryopreservation of a small number of human sperm.