| 高效液相色谱法研究复尔康注射液在家兔体内的药动学特征 |
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| 引用本文： | 刘明华,肖顺汉,何兵,冯文宇,章卓,李蓉,李茂,任美萍.高效液相色谱法研究复尔康注射液在家兔体内的药动学特征[J].四川生理科学杂志,2010,32(1):16-18. |
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| 作者姓名： | 刘明华 肖顺汉 何兵 冯文宇 章卓 李蓉 李茂 任美萍 |
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| 作者单位： | 1. 泸州医学院药理学教研室
2. 泸州医学院药物研究所,四川,泸州,646000 |
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| 基金项目： | 四川省教育厅青年基金项目 |
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| 摘 要： | 目的:建立一种快速的高效液相色谱法(HPLC)同时测定复尔康注射液中维A酸和甘草酸铵的血药浓度,利用3P97药动学软件计算药动学参数,并与维A酸注射液比较其药动学特征。方法:色谱柱:Dikma Kromasil C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液;流速:1.0 mL.min-1;检测波长:分段变波长测定,0～23 min为252 nm,23～40 min为355nm;柱温:30℃;进样量:20μl。结果:复尔康注射液中维A酸和甘草酸铵及维A酸注射液在家兔体内均符合开放二室动力学模型特征。复尔康注射液中维A酸的t1/2α=10.61 min,t1/2β=99.44 min,K10=0.02 min-1,AUC=257.14μg.min.ml-1,CL=0.02mg.kg-1.min-1;维A酸注射液的t1/2α=8.61 min,t1/2β=54.05 min,K10=0.03 min-1,AUC=174.86μg.min.ml-1,CL=0.03 mg.kg-1.min-1;复尔康注射液中甘草酸铵的t1/2α=5.50 min,t1/2β=129.06 min,K10=0.02 min-1,AUC=28611.23μg.min.mL-1,CL=0.01 mg.kg-1.min-1。结论:HPLC简便、快速、精确,可同时检测复尔康注射液中维A酸和甘草酸铵的血药浓度和药动学参数。
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| 关 键 词： | 夏尔康注射液 高效液相色谱法 药动学 维A酸 甘草酸铵 |
Study on the pharmacokinetics of Fuerkang injection in rabbit by HPLC |
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| Liu Ming-hua,Xiao Shun-han,He Bing,Feng Wen-yu,Zhang Zhuo,Li Rong,Li Mao,Ren Mei-ping.Study on the pharmacokinetics of Fuerkang injection in rabbit by HPLC[J].Sichuan Journal of Physiological Sciences,2010,32(1):16-18. |
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| Authors: | Liu Ming-hua Xiao Shun-han He Bing Feng Wen-yu Zhang Zhuo Li Rong Li Mao Ren Mei-ping |
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| Institution: | Liu Ming-hua1,Xiao Shun-han1,He Bing2,Feng Wen-yu2,Zhang Zhuo1,Li Rong1,Li Mao1,Ren Mei-ping1(1.Department of Pharmacology,Pharmacy Faculty,Luzhou Medical College,2.Pharmacy Research Institute,Luzhou 646000) |
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| Abstract: | Objective： To develop a rapid HPLC method for the determination of tretinoin and ammonium glyeyrrhizinate of Fuerkang injection in rabbit plasma, calculate pharmacokinetics parameters by special software Pharmacokinetics Analysis of 3P97, and compare with Tretinoin injection. Method： Dikma Kromasil C18 250 mm 4. 6 mm,5 m）. Mobile phase was acetonitrile- 0. 1% phosphorie acid. Flow rate was 1.0 ml·min^-1. Detection wave length was paragraph and alteration, 0-23min was 252 urn, 23-40min was 355 nm. Clunm temperature was 30℃ and sample size was 20μl. Results. Tretinoin and ammonium glycyrrhizinate of Fuerkang injection and tretinoin injection showed typical characteristics of open two--compartment dynamic model. Tretinoin of Fuerkang injection： t1/2α=10. 61min, t1/2β= 99. 44 min,K10=0. 02 min^-1, AUC=257. 14 μg·min·ml^-1, CL=0.02 mg·kg^-1 ·min^-1. Tretinoin injection： t1/2α=8. 61 min, t1/2β=54.05 min, K10=0. 03 min^-1, AUC=174. 86μg·min·ml^-1 , CL=0. 03 mg · kg^-1 ·min^-1 ; Ammonium glycyrrhizinate of Fuerkang injection： t1/2α=5. 50 min, t1/2β=129. 06 min, K10=0. 02 min^-1 , ALIC=28611. 23μg·min·ml^-1, CL=0. 01 mg kg^-1 min^-1. Conclusion： HPLC method was simple,rapid,accurate,and it can be used to determine simultaneously the plasma concentration and pharmacokinetics parameters of tretinoin and ammonium glycyrrhizinate of Fuerkang injection. |
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| Keywords: | Fuerkang injection HPLC Pharmcokinetics Tretinoin Ammonium glycyrrhizinate |
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