介入化疗对鼻咽癌细胞凋亡和增殖的影响

目的：观察介入化疗对鼻咽癌细胞凋亡（ＡＰＯ）和细胞增殖的影响,并评价其疗效。方法：对１０例鼻咽癌患者经颞浅动脉逆行颈外动脉插管,选择肿瘤供血血管灌注化疗药物５－氟嘧啶、顺铂、平阳霉素及烟酰胺。分别;于化疗前、化疗后７ｄ取肿瘤组织,检测调亡细胞和增殖细胞抗原（ＰＣＮＡ）结果：介入化疗后临床缓解率为６３％。化疗后７ｄ细胞凋亡指数（ＡＩ）１．２４％,高于治疗前的０．５２％。细胞增殖指数化疗前为４２．４％

Timing changes of apoptosis and proliferating cells nuclear antigen after intra-arteiral infusion chemotherapy for nasopharyngeal carcinoma

Objective:To investigate the timing changes of apoptosis (APO) and PCNA after intra-arterial infusion chemotherapy for nasopharyngeal carcinoma.Method:Ten patients were subjected to percutaneous superficial temporal artery catheterization and infusion of anticancer drugs:5 Fu,DDp,BLM, nicotinamide.The biopsy of nasopharyngeal tumor tissues was performed before chemotherapy and 7 days after chemotherapy. Apoptotic cells were examined by TOT mediated DUTP fluorescein and labeling. The expression of proliferating cells nuclear antigen (PCNA) was detected with immunohistochemical staining. Result:AI of nasopharyngeal cancer cells before chemotherapy and 7 days after chemotherapy was 0.52% and 1.42%,PI was 42.4% and 51.2%.Conclusion:Intra arterial infusion chemotherapy not only induced apoptosis effectively, but also inhibited temporarily tumor cells proliferation in patients. The curative surgical treatment should be performed as soon as possible after chemotherapy.