糖肾安改善糖尿病大鼠肾脏病变的作用

目的：研究中药复方糖肾安对糖尿病肾脏病变的改善作用。 方法：Wistar大鼠随机分为4组：对照组（CN），糖尿病组（DM），糖肾安处理的糖尿病组（DM+T）和氨基胍处理的糖尿病组（DM+A）。糖尿病大鼠用糖肾安（6 g?kg^-1）处理8周后，检查肾脏功能和结构的损害程度。 结果：与对照组大鼠比较，糖尿病大鼠血糖、血清肌酐（Scr）和尿素氮（BUN）及尿蛋白排泄量明显升高（P<0.01）。糖肾安处理未能明显降低糖尿病大鼠的血糖水平（P>0.05），但Scr、BUN水平和尿蛋白排泄量明显下降（P<0.01）。给予糖肾安后明显降低糖尿病大鼠已升高的肾小球体积/体重比值。糖尿病大鼠肾小球系膜区PAS阳性染色物质沉积增多，肾小球基底膜呈节段性增厚，上皮细胞足突部分融合，糖肾安处理后这些形态改变明显改善。结论：糖肾安对糖尿病肾脏病变有明显改善作用。

Ameliorative effects of TangShenAn on renal lesions in diabetic rats

Objective To investigate the ameliorative effects of TangShenAn on renal functional and structural lesions in diabetic rats. Methods The Wistar rats were divided into four groups: control group(CN), diabetic group (DM), diabetic rats treated with TangShenAn (DM+T) and diabetic rats treated with aminoguanidine (DM+A). After TangShenAn (6g·kg -1)was administrated to the diabetic rats for 8 weeks, the renal function and structure were evaluated in the animals. Results As compared with controls, the levels of blood glucose, serum creatimine (SCr), blood urea nitrogen (BUN) and urinary protein excretion were significantly elevated in diabetic rats (P<0.01). After the diabetic rats were treated with TangShenAn, the blood glucose level was reduced, but there was no difference between TangShenAn-treated and untreated diabetic animals(P>0.05), while the levels of SCr, BUN and urinary protein excretion were obviously decreased compared with untreated diabetic rats (P<0.01). Administration of TangShenAn decreased the elevated glomerular volume/body weight in diabetic rats. Morphological examination of the renal tissue in diabetic rats exhibited an increased PAS-positive substance in mesangial areas, the segmental thickening of glomerular basement membrane and fusion of epithelial foot processes. After administration of TangShenAn to diabetic rats, these damages were markedly alleviated. Conclusion TangShenAn can ameliorate the renal functional and structural lesions in diabetic rats.