水杨酸钠对大鼠脂肪组织炎症因子表达的影响

目的:探讨水杨酸钠对胰岛素抵抗大鼠胰岛素敏感性的影响及作用机制。方法:分别给大鼠静脉输注脂肪乳,脂肪乳+水杨酸钠和生理盐水7h,并在输注的最后2h,行清醒状态高胰岛素-正血糖钳夹试验,检测脂肪组织中NF-κB及IL-6、TNF-α、SOCS-3、脂联素mRNA的表达。结果:脂肪乳组大鼠脂肪细胞核中NF-κB表达量是生理盐水组2倍,水杨酸钠组NF-κB的表达较脂肪乳组下降35%。脂肪乳组IL-6、TNF-α及SOCS-3 mRNA表达较盐水组升高,输注水杨酸钠炎症因子表达降低。结论:水杨酸钠在脂肪组织内可以抑制NF-κB活化,进而降低炎症因子在脂肪组织蓄积,发挥改善胰岛素抵抗的作用。

Effects of sodium salicylate on the expression of inflammatory cytokines in adipose tissue in insulin resistant rats

Objective：To investigate the mechanisms and effects of sodium salicylate on insulin resistance in rats. Methods： Intralipid （IH）, Sodium Salicylate ＋ Intralipid （SI）, and saline （SAL） were intravenously infused for 7h in rats. During the last 2h of the infusion, insulin sensitivity was measured with glucose infusion rate （GIR） and hyperinsulinemic-euglucemic clamp method. The expressions of NF-κB and IL- 6、TNF-α、SOCS-3 mRNA in white adipose tissues were examined. Results ：During hyperinsulinemic-euglycemic clamp , infusion of intralipid resulted in a 55% with infusion of sodium salicylate; Compared with SAL group, there were elevated 1.9-4 times of the levels of IL-6、TNF-α、SOCS-3 mRNA in adipose tissues in rats with IH, the levels of IL-6、TNF-α、 SOCS-3 mRNA decreased 30%- 50% in SI group. Conclusion： There is activation of NF-κB and downstream eytokines production in white adipose tissue, which causes the insulin resistance both locally and systemically. Sodium salicylate can suppress the activation of NF-κB and downstream cytokine production in adipose tissues and improve insulin resistance.