孕妇HBV-DNA定量对监测母婴HBV传播价值的探讨

目的:对患乙肝母亲和新生儿血清中HBV-DNA定量检测,探讨HBV-DNA定量检测在母婴乙型肝炎病毒宫内传播的价值,指导临床,降低乙肝病毒母婴传播率。方法:采用荧光定量PCR技术对95例乙肝血清标志阳性的孕妇及其婴儿血清进行HBV-DNA定量检测,分析母婴血清中HBV-DNA含量的相关性。结果:“大三阳”组孕妇37例,血清HBV-DNA含量范围为6.00~9.90,均值为7.57±1.27,母婴传播率为91.9%;“小三阳”组孕妇44例,血清HBV-DNA含量范围为<2.00~6.25,均值为4.13±1.64,母婴传播率为52.3%;单一HBcAb阳性组孕妇14例,血清HBV-DNA含量范围为<2.00~4.71,均值为3.17±1.54,母婴传播率为14.3%,HBV母婴宫内传播率随孕妇血清HBV-DNA含量的增高而增加。结论:母婴乙型肝炎病毒宫内传播率与孕妇血清中HBV-DNA含量呈正相关性(r=0.351,P<0.01),当孕妇血清中HBV-DNA含量>6.00时,乙型肝炎病毒母婴宫内传播率显著增加(增加39.3%),提示有效控制患乙肝孕妇血清中HBV-DNA含量,可明显减少宫内母婴乙肝病毒传播率。

Study interrelated of Hepatitis B Virus Transmission from Mothers to Infants with HBV-DNA quantitive PCR

Objective:Examine HBV-DNA content in blood of hepatitis B mothers and their newborn babies,to study interrelated of hepatitis B virus transmission from mothers to their infants with HBV-DNA quantitive PCR.guide treatment,in order to decrease transmission rate of HBV intrauterine transmission.Methods:95 couples of infected HBV pregnant mothers and their newborn babies were detected for HBV by the HBV-DNA fluorescence qualitative PCR and HBV serology.Results:There were 37 couples of infected HBV pregnant mothers who were HBsAg(+),HbeAg(+)and HbcAb(+) and their newborn babies.The HBV-DNA content in the blood of the mothers were from 6.00 to 9.90,the average HBV-DNA content in the mothers was 7.57±1.27.The transmission rate of HBV from mothers to their babies is 91.9%.There were 44 couples of infected HBV pregnant mothers who were HBsAg(+),HbeAb(+)and HbcAb(+) and their newborn babies..The HBV-DNA content in blood of the mothers were from < 2.00 to 6.25,the average HBV-DNA content in the mothers was 4.13±1.64..The transmission rate of HBV from mothers to their babies is 52.3%.There were 14 couples of infected HBV pregnant mothers who were HbcAb(+) and their newborn babies.The HBV-DNA content in blood of the mothers were from < 2.00 to 4.71,the average HBV-DNA content in the mothers was 3.17±1.54.The transmission rata of HBV from mothers to their babies was 14..3%.Result show that the transmission rate of HBV from pregnant mothers to their foetuses was increased in pace with the HBV-DNA content in blood of the pregnant mothers growth.Conclusion:There was positive line interrelate in the HBV-DNA content in umbilical cord blood of infant and the HBV-DNA content in blood of mother(r=0.351,P<0.01).The transmission rate of HBV was markedly increased when the HBV-DNA content in pregnant mothers' serum were > 6.0(increasing 39.3%)..This study showed that the transmission opportunity was marked decrease when the HBV-DNA content in pregnant mothers' serum were decreased.