强次声波对豚鼠Corti器外毛细胞脱氢酶活性的影响

目的 观察强次声波暴露后豚鼠Corti器外毛细胞（OHC）的脱氢酶活性变化及形态变化。方法 将20只豚鼠随机等分为对照组和4个实验组（每组4只）,置于频率8Hz、强度为135dB的次声声场中连续暴露90min。采用“Adobe Photoshop”软件测定豚鼠耳蜗OHC琥珀酸脱氢酶（SDH）显色灰度值（GSV）,比较强次声波暴露前、后8h、2d、5d和21d时OHC的SDH的活性变化,并在光镜下观察耳蜗损伤情况。结果 次声暴露后各组动物耳蜗OHC的SDH显色灰度值明显高于正常对照组（P＜0．01）,光镜下观察发现散在性或局限性OHC肿胀及缺失,细胞境界不清,SDH染色浅谈,且部分OHC的缺失为永久性改变。结论 强次声波能影响OHC的能量代谢,从而明显降低耳蜗OHC的功能,并可导致豚鼠耳蜗OHC永久性的损伤。

Effects of intense infrasound on activities of dehydrogenase of the organ of Corti in guinea pigs

Objective To study the effect of intense infrasound on activities of dehydrogenase and morphorlogical changes of the organ of Corti in guinea pigs. Methods Twenty guinea pigs were randomly divided into five groups: 1 control group and 4 experimental groups.The experimental animals underwent an exposure to 8 Hz infrasound at 135 dB SPL for 90 minutes and were scarified at 8 hours, 2, 5 and 21 days respectively after infrasound exposure. The gray-scale values (GSV) of succinate dehydrogenase (SDH) dyeing were measured by "Adobe Photoshop" software and the organ of Corti were observed under standard light microscope. Results The GSVs of outer hair cell (OHC) of the organ of Corti in 4 experimental groups, especially in 2-day group, were significantly higher than that of control group. Microscopically, restricted or sporadic OHC losses and the swelling of OHC were found from the basal to third cochlear turns. Conclusion Intense infrasound can disturb energy metabolism of the OHC and cause permanent damage of OHC in guinea pigs.