酶联结增强化学发光法(ELECL)诊断血吸虫病的初步研究

本试验采用酶联结增强化学发光法(ELECL)检测日本血吸虫病患者血清中循环抗原,同时就其灵敏度与双抗体夹心ELISA法进行了比较。ELECL法检测血清中循环抗原发光峰值与病人血清稀释度成反比。血清稀释度为1:2560时的发光峰值略低于ELECL阳性判断标准的临界值。同一份标本测定三次,其变异系数为6.7～12.8％。用双抗体夹心ELISA法检测血清中循环抗原,病人血清稀释度为1:40时,其OD值接近ELISA阳性判断标准的临界值。结果表明,ELECL法的灵敏度远较ELISA法高。

ENZYME LINKED ENHANCED CHEMILUMINESCENT(ELECL) FOR DETECTING CIRCULATING ANTIGEN IN HUMAN SCHISTOSOMIASIS JAPONICA

Using enzyme linked enhanced chemiluminescent (ELECL) to identify circulating antigen in sera from patients with schistosomiasis japonica and to compare its sensitivity with sand- wich-ELISA. The results showed that the chemiluminescent peak value(mV) of ELECL re- vealed inverse ratio to the dilution of the patient's serum. The mV was slight lower than the critical-point of ELECL when the serum was diluted to 1:2560. The coefficient of variation (CV) were 6.7-2. 12.8% when one sample was tested repeatedly 3 times. Using sandwich- ELISA to identify the circulating antigen in the sera, its OD value was close to the critical- point of ELISA when patient's serum was diluted to 1 : 40. It suggested that the sensitivity of ELECL was obviously higher than that of ELISA.