IL-18基因105A/C多态性与长江以南汉族人群系统红斑狼疮(SLE)的关联性

 目的  探讨IL-18(interleukin-18)基因105A/C多态性与中国长江以南汉族人群系统性红斑狼疮(systemic lupus erythematosus,SLE)易感性之间的关系。方法  采用病例对照研究设计,收集病例对照各193例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对IL-18基因105位点进行基因分型,在不同的遗传模式下分析105位点基因多态性与SLE的相关性,用χ2检验分析105A/C多态性与SLE临床表型间的相关性。 结果  IL-18基因105位点A等位基因在两组人群中的分布差异有统计学意义(OR=1.815,95% CI=1.151-2.863),在相加和隐性模型下也发现105A/C多态性与SLE有关(相加:OR=1.828,95% CI=1.152-2.900;隐性:OR=1.855,95% CI=1.131-3.044),且调整年龄、性别等因素后,在两种模型下的相关性仍然存在(相加:OR=1.987,95% CI=1.181-3.343;隐性:OR=1.960,95% CI=1.111-3.456)。此外,本研究显示补体C3C4降低患者A等位基因的频率上升(OR=1.737,95% CI=1.001-3.014),但经Bonferroni校正后差异不具有统计学意义( Pcorr = 0.336)。 结论  在中国长江以南汉族人群中IL-18基因105A/C多态性可能与SLE易感性有关,且A等位基因对SLE可能有危险性效应。

Association of interleukin-18 (IL-18) gene 105A/C polymorphism and systemic lupusery erythematosus (SLE) among Chinese Han population in south of the Yangtse River

Objective  To determine the association between interleukin-18 (IL-18) gene polymorphism and the risk of systemic lupus erythematosus(SLE) among Chinese Han population in South of the Yangtze River.MethodsSLE patients (n=193) and healthy individuals (n=193) were recruited for genotyping of the polymorphism 105A/C using the method of restriction fragment length polymorphism (RFLP).The relationship of IL-18 105A/C polymorphism with SLE was analyzed under different genetic models,and the chi-square test was used to explore the association between IL-18 105A/C polymorphism and various clinical features.Results  The frequency of 105A allele was significantly higher in SLE patients (OR=1.815,95%CI=1.151-2.863),and under additive and  recessive model there were significant association between SLE patients and IL-18 105A/C  polymorphism (additive model:OR=1.828,95% CI=1.152-2.900;recessive model:OR=1.855,95% CI=1.131-3.044),even after adjusting covariant,like age and gender,the association still existed (additive model:OR=1.987,95% CI=1.181-3.343;recessive model:OR=1.960,95% CI=1.111-3.456).In addition,this study indicated that patients with lower complement C3 and C4 had higher frequency of 105A allele (OR=1.737,95% CI=1.001-3.014),but P value was not statistically significant after Bonferroni correction (Pcorr = 0.336).Conclusions  The IL-18 gene 105A/C polymorphism is associated with SLE susceptibility in Chinese Han population in South of the Yangtze River,and A allele may be a risk factor for SLE.