脱氧雪腐镰刀菌烯醇,黄曲霉毒素G1对体外培养人外周血淋巴细胞凋？…

以细胞培养、流式细胞义ＤＮＡ含量分析及ＤＮＡ琼脂糖凝胶电泳方法研究了我国食管癌高发区磁县居民粮食中优势污染霉菌毒素脱氧雪腐镰刀菌烯醇（ＤＯＮ）和黄曲霉毒素Ｇ１（ＡＦＧ１）对人淋巴细胞凋亡的影响，流式细胞术（ＦＣＭ）检测结果显示，ＤＯＮ、ＡＦＧ１处理组淋巴细胞出现了典型的亚二倍体凋亡细胞峰，凋亡百分率与毒素作用时间（ＤＯＮ，２－７２小时；ＡＦＧ１；２－２４小时）及测量（ＤＯＮ；５０－２０００μｇ／Ｌ

Effect of deoxynivalenol and aflatoxin G1 on apoptosis of human blood lymphocytes in vitro]

Effect of deoxynivalenol (DON) and aflatoxin G1 (AFG1) on human blood lymphocytes in culture were studied with flow cytometric DNA content analyses and DNA agarose gel electrophoresis. DON and AFG1 are two of the predominant mycotoxins contaminating foodstuffs or residents in a high incidence of esophageal cancer in Cixian County, Hebei province. A typical sub-diploid apoptosis peak was demonstrated in lymphocytes treated with DON and AFG1. A significant dose-effect response and time-effect correlation could be found between apoptosis rates and mycotoxin concentrations (DON: 50-2000 micrograms/L and AFG1: 3.12-2000 micrograms/L) and the treated time (DON: 2-72 hours and AFG1: 2-24 hours). The lymphocytes treated with DON and AFG1 showed characteristic 'ladder' pattern in agarose gel electrophoresis. All results confirmed that DON and AFG1 could induce and accelerate apoptosis in human peripheral blood lymphocytes.