血清谷胱甘肽S-转移酶在小鼠急性硫代乙酰胺肝损伤中的诊断价值

探讨了血清谷胱甘肽Ｓ－转移酶（ＧＳＴ）对硫代乙酰胺（ＴＡＡ）所致小鼠急性肝损伤的早期诊断意义。结果表明，腹腔注射ＴＡＡ２５～１００ｍｇ／ｋｇ能增加小鼠血清ＧＳＴ活性，其活性改变与ＴＡＡ之间存在量－效和时－效关系，与血清ＡＬＴ变化间呈高度正相关。ＴＡＡ致血清ＧＳＴ活性明显异常的同时，肝谷胱甘肽含量和ＧＳＴ、谷胱甘肽过氧化物酶和超氧化物歧化酶活性明显降低。提示肝脏抗氧化功能减退是ＴＡＡ致肝损伤的主要机理之一，血清ＧＳＴ水平是反映ＴＡＡ急性肝损伤的早期灵敏指标。

Serum glutathione S-transferase activity as an early marker of thioacetimide-induced acute hepatotoxicity in mice

The objective of this paper was to evaluate serum glutathione S-transferasethe (GST) in thioacetimide (TAA)-induced hepatotoxicity in mice. The results showed that intraperitoneal injection of TAA (25-100 mg/kg) increased serum GST activity. The activity of GST was dose- and time-related to TAA. There was a good positive correlation between serum GST and serum alanine transaminase(ALT) activities. The content of reduced glutathione(GSH) and activities of GST, glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were significantly decreased while serum GST activity induced by TAA was high. The results suggested that the reduced hepatic antioxidative function is one of the mechanism of TAA-induced hepatotoxicity, and serum GST activity is a sensitive and early marker in detecting TAA-induced hepatotoxicity.