三种不同方法检测β-咔啉类生物碱所致的人胃癌SGC-7901细胞凋亡

目的比较分析目前常用的三种检测细胞凋亡方法在不同β-咔啉类生物碱浓度条件下所致人胃癌SGC-7901细胞凋亡中的运用。方法将生长状态良好的细胞按不同的给药浓度分为A组40μg／ml，B组20μg／ml，C组10μg／ml，D组51xg／ml，E空白对照组，作用24h、48h。分别用MTT法、Hoechest33258细胞核染色法、流式细胞术检测细胞凋亡。结果MTF法结果显示，A组、B组、C组、D组与E组相比较，生长抑制率差异有统计学意义fP〈O．01）；Hoechest33258细胞核染色法显示，E组细胞核浓染、边集、碎裂较少，其他组细胞核均有不同程度的改变，A组、B组、C组、D组与E组相比较，凋亡核百分比差异有统计学意义伙0．05）；流式细胞仪检测结果显示，A组、B组、C组、D组与E组细胞凋亡率相比有统计学差异（P〈0．01）。结论β-咔啉类生物碱能引起人胃癌SGC-7901细胞凋亡。

Three different methods for detection of SGC-7901 cell apoptosis induced by beta-carboline alkaloids

Objective To analyze the detection performance of three methods for SGC-7901 cell apoptosis that induced by beta-carboline alkaloids. Methods SGC-7901 cells were divided into Group A, B, C, D and E. The beta-earboline alkaloids con- eentrations for each group were 40, 20, 10, 5 and 0 p~g/ml respectively. After 24 or 48 hours, cell apoptosis were detected by MTI＇, Hoechest33258 cell nucleus staining method and flow eytometry method. Results The MTI＂ showed that the growth inhibition rate of Group E was significantly different, when compared with Group A,B,C and D （P〈0.01）; Hoechest33258 cell nucleus staining method showed that the percentage of apoptotic nuclear in Groups E was significantly higher than Group A,B,C and D （/9〈0.05）; flow cytometry test showed that the apoptosis rate of Group E was significantly different to Group A,B,C and D （P〈0.01）. Conclu- sion Beta-earboline alkaloids could cause SGC-7901 cell apoptosis.