| Caspase3在多囊卵巢综合征大鼠颗粒细胞的表达及意义 |
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| 引用本文： | 张娟,朱桂金,胡小萍,徐蓓.Caspase3在多囊卵巢综合征大鼠颗粒细胞的表达及意义[J].中国妇幼保健,2011,26(24):3798-3800. |
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| 作者姓名： | 张娟 朱桂金 胡小萍 徐蓓 |
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| 作者单位： | 1. 中国人民解放军第161中心医院妇产科,湖北,武汉,430010
2. 华中科技大学同济医学院附属同济医院生殖中心 |
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| 摘 要： | 目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)信号途径在多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞诱导凋亡的最终效应阶段的作用。方法:采用硫酸普拉睾酮钠诱导大鼠PCOS模型,采用Western blot及RT-PCR分析半胱氨酸天门冬氨酸蛋白酶3(Caspase3)在PCOS大鼠卵巢颗粒细胞的表达情况。结果:①Western blot分析显示,PCOS组大鼠卵巢颗粒细胞活性Caspase3蛋白的表达较对照组明显增强(P<0.01)。②RT-PCR分析显示,PCOS组大鼠卵巢颗粒细胞Caspase3mRNA的表达较对照组明显增强(P<0.01)。结论:TRAIL信号途径在PCOS大鼠颗粒细胞诱导的凋亡可能最终经过了Caspase级联反应,引起Caspase3的激活导致细胞凋亡。
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| 关 键 词： | 多囊卵巢综合征 大鼠 颗粒细胞 Caspase3 |
Expression and significance of Caspase3 in granulosa cells of rats with polycystic ovary syndrome |
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| ZHANG Juan,ZHU Gui-Jin,HU Xiao-Ping et al.Expression and significance of Caspase3 in granulosa cells of rats with polycystic ovary syndrome[J].Maternal and Child Health Care of China,2011,26(24):3798-3800. |
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| Authors: | ZHANG Juan ZHU Gui-Jin HU Xiao-Ping |
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| Institution: | ZHANG Juan,ZHU Gui-Jin,HU Xiao-Ping et al.Department of Gynecology and Obstetrics,PLA No.161 Hospital,Wuhan 430010,Hubei,China |
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| Abstract: | Objective:To explore the role of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) signal pathway in the final effective stage of apoptosis of granulosa cells of rats with polycystic ovary syndrome(PCOS). Methods:Sodium prasterone sulfate was used to induce rat models of PCOS,Western blot and RT-PCR were used to analyze the expression of Caspase3 in granulosa cells of rats with PCOS. Results:Western blot analysis showed that the expression level of actived Caspase3 protein in PCOS group was sig... |
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| Keywords: | Polycystic ovary syndrome Rat Granulosa cell Caspase3 |
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