海口地区138例哮喘患儿血清总IgE水平及过敏原检测分析

目的:探讨海口地区哮喘患儿血清中总IgE水平及主要过敏原种类。方法:采用德国欧蒙印迹法分别检测138例哮喘患儿及40例健康儿童的过敏原种类,并采用美国贝克曼化学发光仪DXI800检测血清中总IgE含量。结果:40例健康儿童血清总IgE含量为116.29±91.60μg/L,过敏原检测均为阴性;138例哮喘患儿总IgE的含量为2 126.17±1 942.69μg/L,138例哮喘患儿总IgE阳性率为73.2%,总IgE水平明显高于对照组过敏原阳性率68.1%(P<0.01),且大多数患儿被多种过敏原致敏。吸入组最高为屋尘57例,其次为屋尘螨/粉尘螨46例;食入组最高为虾20例,其次为鸡蛋白、蟹。结论:通过对哮喘患儿测定血清总IgE水平,并结合过敏原的测定,可了解患儿过敏的变应原,寻找病因,尽量避免接触此类过敏原,减少发病。

The serum level of total IgE and allergen detection and analysis in 138 children with asthma in Haikou region

Objective:To explore the serum level of total IgE and the main kinds of allergens in children with asthma in Haikou region. Methods:Western blot was used to detect the kinds of allergens in 138 children with asthma and 40 healthy children,Beckman chemiluminescence apparatus DXI800 was used to detect the content of total IgE in serum. Results:The content of total IgE in serum of 40 healthy children was(116.29±91.60) μg/L,the result of allergen detection was negative;the content of total IgE in serum of 138 children with asthma was(2 126.17±1 942.69) μg/L,the positive rate of total IgE in 138 children with asthma was 73.2%,which was significantly higher than that in 40 healthy children(68.1%)(P<0.01),most children were sensitized by multiple allergens.In inhalant allergen group,57 children were allergic to house dust,and 45 children were allergic to house dust mites/dust mites;in food allergen group,20 children were allergic to shrimp,followed by egg white and crab. Conclusion:The allergens of children with asthma can be understood by detecting the serum level of total IgE and allergens,searching the causes and avoiding the contact with allergens may reduce the incidence of asthma.