5－羟色胺与体液免疫应答的相互作用

应用定量溶血分光光度测定法（ＱＨＳ）检测５－羟色胺（５－ＨＴ）对大鼠体液免疫应答的影响，并应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠在体液免疫应答的高峰期，中枢和外周５－ＨＴ代谢的变化。结果表明：给大鼠腹腔注射５－ＨＴ的前体５－羟色氨酸（５－ＨＴＰ）后，动物对抗绵羊红细胞（ＳＲＢＣ）的体液免疫应答减弱；腹腔注射５－ＨＴ的合成抑制剂对氯苯丙氨酸（Ｐ－ＣＰＡ）后，免疫应答增强，在体液免疫应答的高峰日（免疫后第５天），下丘脑和脑干内５－ＨＴ的含量明显增加，５－ＨＴ的代谢产物５－羟吲哚乙酸（５－ＨＩＡＡ）的含量无显著变化；脾脏中５－ＨＴ的水平无明显升高，而５－ＨＩＡＡ含量显著增多；海马和胸腺内５－ＨＴ代谢没有明显改变。上述结果提示，５－ＨＴ与体液免疫应答之间存在着相互作用，即５－ＨＴ可能抑制免疫应答，而免疫应答又可加强中枢神经系统和外周胸腺、脾脏内５－ＨＴ的代谢。

INTERACTION BETWEEN SEROTONIN AND HUMORAL IMMUNE RESPONSE

In this study,we determined the effect of 5-hydroxytryptamine(5-HT) on the humoral immune response of rat by the aid of the quantitative hemolysis spoectrophotometry(QHS) and observed the changes of central and peripheral 5-HT metabolism on peak day of the humoral immune response of rat with the help of the high-performance liquid chromatography with electrochemical detection(HPLC-ECD).The results are as follows: Systemic administration of the precursor of serotonin, 5-hydroxytryptoph an(5-UTP),resulted in the suppression of rats to sheep red blood cells(SRBC) response, and injection of para-chlorophenylalanine(p-CPA), which inhibits the biosynthesis of serotonin, markedly enhanced immune response. On day 5 after immunization, the content of 5-HT in the hypothalamus and brainstem significantly increased,and the level of 5-hydroxyindole acetic acid(5-HTAA) which is the metabolite of serotonin was markedly high in the spleen; the 5-HT metabolism in the hippocampus and thymus did not significantly changes. The results suggest that there exists an interaction between the serotonin and humoral immune response, that is,5-HT may inhibit the humoral immune response and the immune response may enhance the central and peripheral 5-HT metabolism.