VES诱导的人口腔鳞癌Tca 8113细胞凋亡作用机制的初步研究

目的：明确维生素E琥珀酸酯（vitamin E succinate，VES）诱导的人口腔鳞癌Tea8113细胞的凋亡作用，初步探讨其凋亡机制。方法：PI单染色和Annexin V-PI双染色后，流式细胞术检测VES对细胞周期的影响及细胞凋亡率；RT-PCR、western blot法检测促凋亡基因bax在mRNA水平和蛋白水平上的表达。结果：VES能显著抑制Tea8113细胞的生长，诱导出现典型的凋亡峰，bax基因及蛋白表达上调。结论：VES能诱导口腔鳞癌细胞Tca8113的凋亡，该作用可能与bax基因表达上调有关。

The action mechanism of the effects of vitamin E succinate on apoptosis of human oral squamous cancer Tca 8113 cells

Objective:To investigate the effect of vitamin E succinate (VES) on apoptosis of Tca 8113 human oral squamous carcinoma cells and its action mechanism. Methods:Flow cytometry was applied to determine the effect of VES on cell cycle distribution and apoptosis after PI staining or Annexin V/PI double staining. The expression of Bax mRNA was quantified by RT-PCR. The relative amounts of the Bax protein was measured by Western blotting. Results:VES significantly inhibited the growth of Tca8113 cells, induced typical apoptosis, and up-regulated the expression of Bax mRNA and protein. After 24 h the apoptotic rate reached the peak level. Conclusion:VES induces apoptosis of Tca8113 human oral squamous carcinoma cells, which is probably related with up-regulation of Bax expression.