肝癌模型冷冻治疗前后血清AFP,IL-10和TGF-β1水平的测定及意义

目的小鼠肝细胞癌模型经冷冻处理后,通过测量外周血AFP值及IL-10、TGFβ1的浓度,研究小鼠肝癌经冷冻处理后的体内免疫状况的变化.方法取Balb/s小鼠48只制成肝癌模型,随机分为2组,一组为对照组,另一组为冷冻处理组,分别于冷冻处理后2 h、2 W、3 W、4 W采用眼球取血法取血,采用放免法测AFP值,采用双抗体夹心ELISA法测其血浆中IL-10、TGF-β1的浓度.比较两组小鼠治疗前后免疫功能的变化情况.结果冷冻组各指标呈递减趋势,与对照组比较差别有显著性,各时间点组间比较差别有显著性.结论小鼠肝细胞癌模型经冷冻治疗术后免疫功能增强,存在显著的冷冻免疫效应.

Detection and significance of levels of AFP,IL-10,TGF-β1 before and after cryosurgery of HCC model

Objective: To study immunocompetent changes in mice after cryosurgical treatment for liver cancer and provide immunological evidences for clinical application of cryosurgery when levels of AFP, TGF - β1 and IL-10 were checked in periphery blood of HCC model. Methods :3 - weeks - old Balb mice were randomized into control group and cryosurgery group after injection of HCC Hela cells 2 × 104/mice into abdoiminal cavity. Female and male mice of every group were all 24. Tumors were apparently upgrowth at injection position on 14 days in all mice. Average diameter was 0.8 cm and the biggest one was 1.6 cm. Blood specimens were acquired by eyeball blood sampling to test relative immune criteria in 2 h, 14,21,28 days after management. ELISA was applied to measure the changes of IL - 10 and TGF - β1 and compared immunocompetent changes in two groups. Results:Immune criterion measurement indicated that levels of IL- 10 ,TGF - β1 and AFP decreased in cryosurgery group, and there was significant difference between control group and cryosurgery group. Conclusions: Immunoenhancement could be achieved in HCC after cryosurgery and cryoimmune effects are presented.